Literature summary for 3.4.17.2 extracted from

Edge, M.; Forder, C.; Hennam, J.; Lee, I.; Tonge, D.; Hardern, I.; Fitton, J.; Eckersley, K.; East, S.; Shufflebotham, A.; Blakey, D.; Slater, A.
Engineered human carboxypeptidase B enzymes that hydrolyse hippuryl-L-glutamic acid: reversed-polarity mutants (1998), Protein Eng., 11, 1229-1234.

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Cloned(Commentary)

Cloned (Comment)	Organism
-	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
D253K	the mutant enzyme is unable to hydrolyze hippuryl-Arg. Activity with hippuryl-Glu and hippuryl-Asp, this activity is reduced hundred-fold compared with the native enzyme with hippuryl-Arg as substrate	Homo sapiens
D253R	the mutant enzyme is unable to hydrolyze hippuryl-Arg. Activity with hippuryl-Glu and hippuryl-Asp, this activity is reduced hundred-fold compared with the native enzyme with hippuryl-Arg as substrate	Homo sapiens
G251T/D253K	the mutant enzyme is unable to hydrolyze hippuryl-Arg. 100times more active against hippuryl-L-Glu as substrate than the single mutant D253K	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	Km-values of mutant enzymes with hippuryl-Glu as substrate	Homo sapiens
0.18	-	hippuryl-L-Arg	-	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	turnover-number of mutant enzymes with hippuryl-Glu	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
hippuryl-L-Arg	mutant enzymes S253K and S253R are inactive	Homo sapiens	hippuric acid + L-Arg	-	?
hippuryl-L-Asp + H2O	hydrolyzed by mutant enzyme D253K and mutant enzyme D253R, wild type enzyme shows no activity	Homo sapiens	hippuric acid + L-aspartate	-	?
hippuryl-L-Glu + H2O	hydrolyzed by mutant enzyme D253K, wild type enzyme shows no activity	Homo sapiens	hippuric acid + Glu	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.16	-	hippuryl-Asp	mutant enzyme D253R	Homo sapiens
0.26	-	hippuryl-Asp	mutant enzyme D253K	Homo sapiens
3.8	-	hippuryl-Glu	mutant enzyme D253K	Homo sapiens
61	-	hippuryl-Arg	-	Homo sapiens

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Release 2023.1 (January 2023)