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Literature summary for 3.4.17.19 extracted from
- Two metallocarboxypeptidases from the protozoan Trypanosoma cruzi belong to the M32 family, found so far only in prokaryotes (2007), Biochem. J., 401, 399-410.
Application
| Application | Comment | Organism |
|---|---|---|
| drug development | potential target for drug development | Trypanosoma cruzi |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli XL1 Blue cells as catalytically active poly-His-tagged recombinant enzyme. GST-fusion proteins expressed in Escherichia coli BL21 Codon Plus (DE3) cells | Trypanosoma cruzi |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | at 1 mM 92.2% activity relative to control | Trypanosoma cruzi |  |
| Co2+ | at 0.01 mM 59.7% activity relative to control | Trypanosoma cruzi | |
| EDTA | 10 mM strongly inhibits | Trypanosoma cruzi | |
| Mg2+ | at 1 mM 88% activity relative to control | Trypanosoma cruzi | |
| Mn2+ | at 0.1 mM 76% activity relative to control | Trypanosoma cruzi | |
| additional information | PMSF at 2 mM and E-64 at 0.001 mM have no effect on activity | Trypanosoma cruzi | |
| Ni2+ | at 0.01 mM 44.6% activity relative to control | Trypanosoma cruzi | |
| o-phenanthroline | 1 mM strongly inhibits | Trypanosoma cruzi | |
| Zn2+ | at 0.01 mM 8.98% activity relative to control | Trypanosoma cruzi | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.073 | - |
N-(3-[2-furyl]acryloyl)-Phe-Phe | in the presence of Co2+ | Trypanosoma cruzi | |
| 0.166 | - |
N-(3-[2-furyl]acryloyl)-Ala-Lys | in the absence of Co2+ | Trypanosoma cruzi | |
| 0.169 | - |
N-(3-[2-furyl]acryloyl)-Ala-Lys | in the presence of Co2+ | Trypanosoma cruzi | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Trypanosoma cruzi | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57700 | - |
x * 57700, sequence analysis, x * 59000, Western blot analysis | Trypanosoma cruzi |
| 59000 | - |
x * 57700, sequence analysis, x * 59000, Western blot analysis | Trypanosoma cruzi |
| 128000 | - |
gel filtration | Trypanosoma cruzi |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trypanosoma cruzi | Q6ZXC0 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| gel filtration and two ion-exchange chromatography steps (Ni- and Co-NTA resin), 20.4fold purification and 32% recovery | Trypanosoma cruzi |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| amastigote | - |
Trypanosoma cruzi | - |
| epimastigote | - |
Trypanosoma cruzi | - |
| additional information | present in all life cycle stages | Trypanosoma cruzi | - |
| trypomastigote | cell-derived and metacylic trypomastigotes | Trypanosoma cruzi | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.2 | - |
cell-free extract | Trypanosoma cruzi |
| 85 | - |
20.4fold purified enzyme | Trypanosoma cruzi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | MCP-1 acts best on substrates having a basic amino acid at P1' position. Activity is very low with hydrophobic C-terminal residues | Trypanosoma cruzi | ? | - |
? | |
| N-(3-[2-furyl]acryloyl)-Ala-Lys + H2O | - |
Trypanosoma cruzi | ? | - |
? | |
| N-(3-[2-furyl]acryloyl)-Phe-Phe + H2O | - |
Trypanosoma cruzi | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 57700, sequence analysis, x * 59000, Western blot analysis | Trypanosoma cruzi |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MCP-1 | - |
Trypanosoma cruzi |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
residual activity after 5 min of incubation at 50°C is 21.4%, the enzyme is not thermostable | Trypanosoma cruzi |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.7 | - |
N-(3-[2-furyl]acryloyl)-Phe-Phe | in the presence of Co2+ | Trypanosoma cruzi | |
| 5.7 | - |
N-(3-[2-furyl]acryloyl)-Ala-Lys | in the presence of Co2+ | Trypanosoma cruzi | |
| 20 | - |
N-(3-[2-furyl]acryloyl)-Ala-Lys | in the absence of Co2+ | Trypanosoma cruzi | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.2 | - |
- |
Trypanosoma cruzi |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.2 | 7.3 | - |
Trypanosoma cruzi |
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