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Literature summary for 3.4.17.19 extracted from

  • Lee, S.H.; Minagawa, E.; Taguchi, H.; Matsuzawa, H.; Ohta, T.; Kaminogawa, S.; Yamauchi, K.
    Purification and characterization of a thermostable carboxypeptidase (carboxypeptidase Taq) from Thermus aquaticus YT-1 (1992), Biosci. Biotechnol. Biochem., 56, 1839-1844.
    View publication on PubMed
Search for more literature for 3.4.17.19

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
 Thermus aquaticus
EDTA
-
 Thermus aquaticus
guanidine hydrochloride
-
 Thermus aquaticus
Metal chelating reagents
-
 Thermus aquaticus
SDS
-
 Thermus aquaticus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 50% of the activation with Co2+ Thermus aquaticus
Co2+ dependent on Thermus aquaticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
 1 * 56000, Thermus aquaticus, SDS-PAGE Thermus aquaticus
58000
-
 Thermus aquaticus, gel filtration Thermus aquaticus

Organism

Organism UniProt Comment Textmining
Thermus aquaticus
-
 YT-1
-
Thermus aquaticus YT-1
-
 YT-1
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermus aquaticus

Storage Stability

Storage Stability Organism
-20°C Thermus aquaticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Carbobenzoxy-Gly-Ala + H2O
-
 Thermus aquaticus Carbobenzoxy-Gly + Ala
-
 ?
Carbobenzoxy-Gly-Ala + H2O
-
 Thermus aquaticus YT-1 Carbobenzoxy-Gly + Ala
-
 ?
Carbobenzoxy-Gly-Leu + H2O
-
 Thermus aquaticus Carbobenzoxy-Gly + Leu
-
 ?
Carbobenzoxy-Gly-Leu + H2O
-
 Thermus aquaticus YT-1 Carbobenzoxy-Gly + Leu
-
 ?
Carbobenzoxy-Gly-Phe + H2O
-
 Thermus aquaticus Carbobenzoxy-Gly + Phe
-
 ?
Carbobenzoxy-Gly-Phe + H2O
-
 Thermus aquaticus YT-1 Carbobenzoxy-Gly + Phe
-
 ?
Carbobenzoxy-Gly-Pro-Leu-Gly + H2O
-
 Thermus aquaticus Carbobenzoxy-Gly-Pro-Leu + Gly
-
 ?
Carbobenzoxy-Gly-Val + H2O
-
 Thermus aquaticus Carbobenzoxy-Gly + Val
-
 ?
Carbobenzoxy-Gly-Val + H2O
-
 Thermus aquaticus YT-1 Carbobenzoxy-Gly + Val
-
 ?
additional information enzyme releases amino acids sequentially from the C-terminus, broad substrate specificity, although proline at the C-terminus of peptides is not cleaved Thermus aquaticus ?
-
 ?
additional information enzyme releases amino acids sequentially from the C-terminus, broad substrate specificity, although proline at the C-terminus of peptides is not cleaved Thermus aquaticus YT-1 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 56000, Thermus aquaticus, SDS-PAGE Thermus aquaticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
 Thermus aquaticus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 90 30°C: less than 30% of activity maximum, 90°C: more than 90% of activity maximum Thermus aquaticus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 high thermostability is independent of Co2+ Thermus aquaticus
4
-
 pH 7-10, 18 h, stable Thermus aquaticus
25
-
 pH 7-9, 5 h, stable Thermus aquaticus
80
-
 5 h, 10% loss of activity Thermus aquaticus
90
-
 5 h, about 80% loss of activity Thermus aquaticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Thermus aquaticus

pH Range

pH Minimum pH Maximum Comment Organism
6 8.5 pH 6: about 35% of activity maximum, pH 8.5: about 85% of activity maximum Thermus aquaticus

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 9 25°C, 5 h, stable Thermus aquaticus
7 10 4°C, 18 h, stable Thermus aquaticus