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Literature summary for 3.4.17.18 extracted from
- Structural principles of the broad substrate specificity of Thermoactinomyces vulgaris carboxypeptidase T--role of amino acid residues at positions 260 and 262 (2008), Protein Eng. Des. Sel., 21, 545-551.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into the vector pET23a for expression in Escherichia coli BL21DE3 pLysS cells | Thermoactinomyces vulgaris |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D260G | mutant, the influence of residues at positions 260 and 262 on a broad substrate specificity is studied | Thermoactinomyces vulgaris |
| D260G/T262I | mutant, the influence of residues at positions 260 and 262 on a broad substrate specificity is studied | Thermoactinomyces vulgaris |
| D260G/T262K | mutant, the influence of residues at positions 260 and 262 on a broad substrate specificity is studied | Thermoactinomyces vulgaris |
| D260G/T262R | mutant, the influence of residues at positions 260 and 262 on a broad substrate specificity is studied | Thermoactinomyces vulgaris |
| G215S/A251G/D253_L254insT/T257A/D260G/T262D | mutant CPT6 | Thermoactinomyces vulgaris |
| G215S/A251G/T257A/D260G/T262D | mutant CPT5 | Thermoactinomyces vulgaris |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.016 | - |
N-benzyloxycarbonyl-Ala-Ala-Leu | mutant CPT D260G | Thermoactinomyces vulgaris |  |
| 0.03 | - |
N-benzyloxycarbonyl-Ala-Ala-Leu | mutant CPT D260G/T261I | Thermoactinomyces vulgaris | |
| 0.033 | - |
N-benzyloxycarbonyl-Ala-Ala-Leu | mutant CPT D260G/T262K | Thermoactinomyces vulgaris | |
| 0.046 | - |
N-benzyloxycarbonyl-Ala-Ala-Leu | CPT wild-type | Thermoactinomyces vulgaris | |
| 0.15 | - |
N-benzyloxycarbonyl-Ala-Ala-Leu | mutant CPT5 | Thermoactinomyces vulgaris | |
| 0.18 | - |
N-benzyloxycarbonyl-Ala-Ala-Glu | mutant CPT D260G | Thermoactinomyces vulgaris | |
| 0.3 | - |
N-benzyloxycarbonyl-Ala-Ala-Glu | mutant CPT D260G/T262I | Thermoactinomyces vulgaris | |
| 0.45 | - |
N-benzyloxycarbonyl-Ala-Ala-Leu | mutant CPT D260G/T262R | Thermoactinomyces vulgaris | |
| 0.65 | - |
N-benzyloxycarbonyl-Ala-Ala-Glu | mutant CPT D260G/T262K | Thermoactinomyces vulgaris | |
| 0.75 | - |
N-benzyloxycarbonyl-Ala-Ala-Leu | mutant CPT6 | Thermoactinomyces vulgaris | |
| 0.8 | - |
N-benzyloxycarbonyl-Ala-Ala-Glu | mutant CPT D260G/T262R | Thermoactinomyces vulgaris | |
| 2.8 | - |
N-2,4-dinitrophenyl-Ala-Ala-Arg | mutant CPT D260G | Thermoactinomyces vulgaris | |
| 4.4 | - |
N-2,4-dinitrophenyl-Ala-Ala-Arg | CPT wild-type | Thermoactinomyces vulgaris | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Thermoactinomyces vulgaris | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermoactinomyces vulgaris | P29068 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by gel filtration on a Superdex TM 75 column and by using a CABS-Sepharose column | Thermoactinomyces vulgaris |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| refolding after treatment with 8 M urea | Thermoactinomyces vulgaris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-2,4-dinitrophenyl-Ala-Ala-Arg + H2O | - |
Thermoactinomyces vulgaris | N-2,4-dinitrophenyl-Ala-Ala-Arg + L-arginine | - |
? | |
| N-benzyloxycarbonyl-Ala-Ala-Glu + H2O | - |
Thermoactinomyces vulgaris | N-benzyloxycarbonyl-Ala-Ala + L-glutamate | - |
? | |
| N-benzyloxycarbonyl-Ala-Ala-Leu + H2O | - |
Thermoactinomyces vulgaris | N-benzyloxycarbonyl-Ala-Ala + L-leucine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| carboxypeptidase T | - |
Thermoactinomyces vulgaris |
| CPT | - |
Thermoactinomyces vulgaris |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
hydrolysis of N-benzyloxycarbonyl-Ala-Ala-Leu and N-benzyloxycarbonyl-Ala-Ala-Glu | Thermoactinomyces vulgaris |
| 37 | - |
hydrolysis of N-2,4-dinitrophenyl-Ala-Ala-Arg | Thermoactinomyces vulgaris |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.04 | - |
N-2,4-dinitrophenyl-Ala-Ala-Arg | mutant CPT D260G | Thermoactinomyces vulgaris | |
| 1.6 | - |
N-benzyloxycarbonyl-Ala-Ala-Leu | mutant CPT D260G/T262K | Thermoactinomyces vulgaris | |
| 4.1 | - |
N-benzyloxycarbonyl-Ala-Ala-Leu | mutant CPT D260G | Thermoactinomyces vulgaris | |
| 5.2 | - |
N-benzyloxycarbonyl-Ala-Ala-Leu | mutant CPT D260G/T261I | Thermoactinomyces vulgaris | |
| 5.6 | - |
N-benzyloxycarbonyl-Ala-Ala-Leu | mutant CPT D260G/T262R | Thermoactinomyces vulgaris | |
| 7.1 | - |
N-benzyloxycarbonyl-Ala-Ala-Leu | mutant CPT6 | Thermoactinomyces vulgaris | |
| 7.2 | - |
N-benzyloxycarbonyl-Ala-Ala-Glu | mutant CPT D260G | Thermoactinomyces vulgaris | |
| 8.5 | - |
N-benzyloxycarbonyl-Ala-Ala-Glu | mutant CPT D260G/T262I | Thermoactinomyces vulgaris | |
| 10.3 | - |
N-benzyloxycarbonyl-Ala-Ala-Leu | CPT wild-type | Thermoactinomyces vulgaris | |
| 11.5 | - |
N-benzyloxycarbonyl-Ala-Ala-Glu | mutant CPT D260G/T262K | Thermoactinomyces vulgaris | |
| 14.6 | - |
N-2,4-dinitrophenyl-Ala-Ala-Arg | CPT wild-type | Thermoactinomyces vulgaris | |
| 22.8 | - |
N-benzyloxycarbonyl-Ala-Ala-Leu | mutant CPT5 | Thermoactinomyces vulgaris | |
| 23.6 | - |
N-benzyloxycarbonyl-Ala-Ala-Glu | mutant CPT D260G/T262R | Thermoactinomyces vulgaris | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
hydrolysis of N-2,4-dinitrophenyl-Ala-Ala-Arg, N-benzyloxycarbonyl-Ala-Ala-Leu and N-benzyloxycarbonyl-Ala-Ala-Glu | Thermoactinomyces vulgaris |
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