Literature summary for 3.4.17.18 extracted from

Trachuk, L.; Letarov, A.; Kudelina, I.A.; Yusupova, M.P.; Chestukhina, G.G.
In vitro refolding of carboxypeptidase T precursor from Thermoactinomyces vulgaris obtained in Escherichia coli as cytoplasmic inclusion bodies (2005), Protein Expr. Purif., 40, 51-59.

Search for more literature for 3.4.17.18

Activating Compound

Activating Compound	Comment	Organism	Structure
Subtilisin	cleavage of the carboxypeptidase precursor	Thermoactinomyces vulgaris

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Thermoactinomyces vulgaris

General Stability

General Stability	Organism
Ca2+ important for thermostability	Thermoactinomyces vulgaris

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	10 mM, required for enzyme stabilization	Thermoactinomyces vulgaris

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
38000	-	SDS-PAGE, mature enzyme	Thermoactinomyces vulgaris

Organism

Organism	UniProt	Comment	Textmining
Thermoactinomyces vulgaris	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
purification from inclusion bodes	Thermoactinomyces vulgaris

Renatured (Commentary)

Renatured (Comment)	Organism
best conditions: 30 mM Tris-HCl pH 9.0, 0.5 M NaCl, 30% glycerol, 2 mM dithiothreitol, 2 mM cysteine, 10 mM CaCl2, 40°C for 12 h	Thermoactinomyces vulgaris

Source Tissue

Source Tissue	Comment	Organism	Textmining
cell culture	-	Thermoactinomyces vulgaris	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
8.2	-	0.2 ml 50 mM Tris-HCl pH 8.0, 5 mM CaCl2, 0.2 ml 2,4-dinitrophenyl-Ala-Ala-Arg, 37°C	Thermoactinomyces vulgaris

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2,4-dinitrophenyl-Ala-Ala-Arg + H2O	-	Thermoactinomyces vulgaris	2,4-dinitrophenyl-Ala-Ala + Arg	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 38000, SDS-PAGE, mature enzyme	Thermoactinomyces vulgaris

Synonyms

Synonyms	Comment	Organism
carboxypeptidase T	-	Thermoactinomyces vulgaris
CPT	-	Thermoactinomyces vulgaris

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
65	-	10% denatured protein after 2 h at 65°C, 30 mM Tris-HCl pH 9.0, 0.5 M NaCl, 30% glycerol, 2 mM dithiothreitol, 2 mM cysteine, 10 mM CaCl2, rapid denaturing occurs without CaCl2 under the same conditions	Thermoactinomyces vulgaris

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Release 2023.1 (January 2023)