Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, functional expression in Escherichia coli | Pseudomonas aeruginosa |
Crystallization (Comment) | Organism |
---|---|
purified recombinant wild-type enzyme and mutants S115A and H285A, sitting drop vapour diffusion method, 0.004 ml of 6 mg/ml protein containing solution is mixed with an equal volume of reservoir solution containing 20 mM CaCl2 dihydrate, 0.1 M sodium acetate trihydrate, pH 4.6, and 30% v/v 2-methyl-2,4-pentanediol, room temperature, 6 mg/ml selenomethionine-labeled enzyme from 50 mM citric acid, pH 4.5, 21°C, X-ray diffraction structure determination and analysis at 1.5-2.4 A resolution | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
E217A | site-directed mutagenesis, nearly inactive mutant, lack of activity might possibly be due to a folding defect, no crystallization of the mutant enzyme | Pseudomonas aeruginosa |
H285A | site-directed mutagenesis, nearly inactive mutant, lack of activity is not due to a folding defect | Pseudomonas aeruginosa |
S115A | site-directed mutagenesis, nearly inactive mutant, lack of activity is not due to a folding defect | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
nocardicin A | - |
Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34600 | - |
2 * 34600, about, sequence calculation | Pseudomonas aeruginosa |
51000 | - |
recombinant enzyme, high salt condition gel filtration | Pseudomonas aeruginosa |
56000 | - |
recombinant enzyme, low salt condition gel filtration | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
peptidoglycan + H2O | Pseudomonas aeruginosa | the enzyme is involved in peptidoglycan recycling | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | Q9HTZ1 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli | Pseudomonas aeruginosa |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
hydrolysis of the bond: N-acetyl-D-glucosaminyl-N-acetylmuramoyl-L-Ala-D-glutamyl-6-carboxy-L-lysyl-/-D-alanine | the enzyme is a serine peptidase with a Ser115-His285-Glu217 catalytic triad | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GlcNAc-MurNAc tetrapeptide + H2O | substrate prepared from purified murein by lysozyme, cleaves specifically between meso-diaminopimelic acid and D-alanine | Pseudomonas aeruginosa | ? | - |
? | |
additional information | the enzyme is a serine peptidase which cleaves between the L- and D-amino acids of bacterial peptidoglycan | Pseudomonas aeruginosa | ? | - |
? | |
peptidoglycan + H2O | the enzyme is involved in peptidoglycan recycling | Pseudomonas aeruginosa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 34600, about, sequence calculation | Pseudomonas aeruginosa |
More | the enzyme consists of an N-terminal beta-sheet and a C-terminal beta-barrel domain, at the interface of the two domains Ser115 adopts a highly strained conformation in a strand-turn-helix motif similar to the nucleophilic elbow in alphabeta-hydrolases, domain structure, overview | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
LD-Carboxypeptidase | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Pseudomonas aeruginosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | total turnover of wild-type and mutant enzymes in comparison | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Pseudomonas aeruginosa |