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Literature summary for 3.4.17.12 extracted from
- Carboxypeptidase M (2004), Handbook of Proteolytic Enzymes (Barrett, J. ; Rawlings, N. D. ; Woessner, J. F. , eds. ) Academic Press, 2, 851-854.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, genetic structure, localization on chromosome 12q13-q15, expression in insect cells via baculovirus transfection | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Homo sapiens |  |
| Cadmium acetate | - |
Homo sapiens | |
| DL-2-mercaptomethyl-3-guanidinoethylthiopropanoic acid | inhibits best at acidic pH | Homo sapiens | |
| Guanidinoethylmercaptosuccinic acid | - |
Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cell surface | - |
Homo sapiens | 9986 | - |
| cell surface | - |
Canis lupus familiaris | 9986 | - |
| extracellular | - |
Homo sapiens | - |
- |
| extracellular | - |
Canis lupus familiaris | - |
- |
| membrane | bound | Canis lupus familiaris | 16020 | - |
| plasma membrane | anchored via glycosylphosphatidylinositol | Homo sapiens | 5886 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | activates, best at acidic pH | Homo sapiens | |
| additional information | the enzyme is a metallopeptidase | Homo sapiens |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47600 | - |
1 * 62000, glycosylated enzyme, SDS-PAGE, 1 * 47600, deglycosylated enzyme, SDS-PAGE | Homo sapiens |
| 62000 | - |
1 * 62000, glycosylated enzyme, SDS-PAGE, 1 * 47600, deglycosylated enzyme, SDS-PAGE | Homo sapiens |
| 73000 | - |
gel filtration | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| epidermal growth factor + H2O | Homo sapiens | - |
dea-Arg53-epidermal growth factor + arginine | - |
? | |
| additional information | Homo sapiens | the enzyme controls the activity of kinins in the kidney, is involved in inflammation, and participates in the metabolism of growth factors that contain C-terminal Arg or Lys residues, e.g. the epidermal growth factor, the nerve growth factor, the hypatocyte growth factor, the macrophage-stimulating protein, or erythropoietin | ? | - |
? | |
| additional information | Canis lupus familiaris | the enzyme may be responsible for control of kinins and epidermal growth factor as well as of EGF-like peptides | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Canis lupus familiaris | - |
- |
- |
| Homo sapiens | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | the enzyme contains 6 potential glycosylation sites and 23% carbohydrate content | Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme from placental microvillus membranes by solubilization through either phosphatidylinositol-specific phospholipase C or CHAPS, ion exchange and arginine affinity chromatography, and gel filtration | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| amniotic fluid | - |
Canis lupus familiaris | - |
| blood vessel | - |
Homo sapiens | - |
| brain | - |
Homo sapiens | - |
| intestine | - |
Homo sapiens | - |
| kidney | - |
Homo sapiens | - |
| kidney | synthesized and released in distal tubules | Canis lupus familiaris | - |
| lung | - |
Homo sapiens | - |
| MDCK cell | Madnine-Darby canine kidney cells, high expression level | Canis lupus familiaris | - |
| nerve | peripheral | Homo sapiens | - |
| placenta | microvillus | Homo sapiens | - |
| urine | - |
Canis lupus familiaris | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| epidermal growth factor + H2O | - |
Homo sapiens | dea-Arg53-epidermal growth factor + arginine | - |
? | |
| additional information | the enzyme controls the activity of kinins in the kidney, is involved in inflammation, and participates in the metabolism of growth factors that contain C-terminal Arg or Lys residues, e.g. the epidermal growth factor, the nerve growth factor, the hypatocyte growth factor, the macrophage-stimulating protein, or erythropoietin | Homo sapiens | ? | - |
? | |
| additional information | the enzyme may be responsible for control of kinins and epidermal growth factor as well as of EGF-like peptides | Canis lupus familiaris | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 62000, glycosylated enzyme, SDS-PAGE, 1 * 47600, deglycosylated enzyme, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CPM | - |
Homo sapiens |
| CPM | - |
Canis lupus familiaris |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
neutral pH-optimum | Homo sapiens |
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