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Literature summary for 3.4.17.12 extracted from

  • Craveiro, R.B.; Ramalho, J.D.; Chagas, J.R.; Wang, P.H.; Casarini, D.; Pesquero, J.L.; Araujo, R.C.; Pesquero, J.B.
    High expression of human carboxypeptidase M in Pichia pastoris: purification and partial characterization (2006), Braz. J. Med. Biol. Res., 39, 211-217.
    View publication on PubMed
Search for more literature for 3.4.17.12

Application

Application Comment Organism
medicine CPM might be an important target for the development of new drugs to treat inflammatory diseases and cancer Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Homo sapiens
PCR-amplified fragment encoding hCPM is cloned into the pGEM-T-Easy cloning vector, and subsequent into the pPIC9 vector for transfomation of Pichia pastoris cells Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Homo sapiens 16020
-
membrane anchored via glycosylphosphatidylinositol Homo sapiens 16020
-
soluble
-
 Homo sapiens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+
-
 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
 determined by SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Homo sapiens
glycoprotein glycosylphosphatidylinositol-anchored protein Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme 1753fold from Pichia pastoris strain GS115 by gel filtration and anion exchange chromatography to homogeneity Homo sapiens
the CPM secreted in the supernatant is purified by a two-step procedure consisting of gel filtration and ion-exchange chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
amniotic fluid
-
 Homo sapiens
-
blood vessel
-
 Homo sapiens
-
brain
-
 Homo sapiens
-
intestine
-
 Homo sapiens
-
kidney
-
 Homo sapiens
-
lung
-
 Homo sapiens
-
peripheral nerve
-
 Homo sapiens
-
placenta
-
 Homo sapiens
-
seminal plasma
-
 Homo sapiens
-
urine
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 purified recombinant enzyme, relative fluorescent units Homo sapiens
additional information
-
 16474, RFU min-1 mg-1, purification step Mono Q, RFU - relative fluorescence units Homo sapiens
additional information
-
 2415 RFU min-1 mg-1, purification step Superdex 200 HR, RFU - relative fluorescence units Homo sapiens
additional information
-
 9.4 RFU min-1 mg-1, purification step supernatant, RFU - relative fluorescence units Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Dansyl-Ala-Arg + H2O
-
 Homo sapiens Dansyl-Ala + Arg
-
 ?
Dansyl-Ala-Arg + H2O substrate used in activity assay Homo sapiens Dansyl-Ala + Arg
-
 ?
additional information removing of the C-terminal Arg residue of the intact kinins and related peptides Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
? x * 62000-70000, recombinant enzyme, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
carboxypeptidase M
-
 Homo sapiens
CPM
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 neutral pH Homo sapiens