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Literature summary for 3.4.17.12 extracted from

  • Skidgel, R.A.; Davis, R.M.; Tan, F.
    Human carboxypeptidase M. Purification and characterization of a membrane-bound carboxypeptidase that cleaves peptide hormones (1989), J. Biol. Chem., 264, 2236-2241.
    View publication on PubMed
Search for more literature for 3.4.17.12

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
 Homo sapiens
2-mercaptomethyl-3-guanidinoethyl thiopropanoic acid
-
 Homo sapiens
Cd(CH3COO)2
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.016
-
 bradykinin
-
 Homo sapiens
0.046
-
 Met5-Arg6-enkephalin
-
 Homo sapiens
0.063
-
 Leu5-Arg6-enkephalin
-
 Homo sapiens
0.375
-
 Met5-Lys6-enkephalin
-
 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane
-
 Homo sapiens 5886
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ CoCl2 activates Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
 x * 62000, human, SDS-PAGE with or without reduction Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens the enzyme could inactivate or modulate the activity of peptide hormones either before or after their interaction with plasma membrane receptors ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein carbohydrate content: 23% by weight Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
placenta microvilli Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzoyl-Ala-Lys + H2O
-
 Homo sapiens benzoyl-Ala + Lys
-
 ?
benzoyl-Gly-Arg + H2O
-
 Homo sapiens benzoyl-Gly + Arg
-
 ?
benzoyl-Gly-Lys + H2O
-
 Homo sapiens benzoyl-Gly + Lys
-
 ?
bradykinin + H2O
-
 Homo sapiens ?
-
 ?
Dansyl-Ala-Arg + H2O
-
 Homo sapiens Dansyl-Ala + Arg
-
 ?
dynorphin A(1-13) + H2O i.e. Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg-Pro-Lys-Leu-Lys Homo sapiens Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg-Pro-Lys-Leu + Lys
-
 ?
Leu5-Arg6-enkephalin + H2O
-
 Homo sapiens ?
-
 ?
Met5-Arg6-enkephalin + H2O
-
 Homo sapiens ?
-
 ?
Met5-Lys6-enkephalin + H2O
-
 Homo sapiens ?
-
 ?
additional information carboxypeptidase M does not hydrolyze the peptidyl dipeptidase substrate furylacryloyl-Phe-Gly-Gly or the carboxypeptidase A substrates benzoyl-Gly-phenyllactic acid and furylacryloyl-Phe-Phe Homo sapiens ?
-
 ?
additional information the enzyme could inactivate or modulate the activity of peptide hormones either before or after their interaction with plasma membrane receptors Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
? x * 62000, human, SDS-PAGE with or without reduction Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.77
-
 Leu5-Arg6-enkephalin
-
 Homo sapiens
2.45
-
 bradykinin
-
 Homo sapiens
11.1
-
 Met5-Lys6-enkephalin
-
 Homo sapiens
15.6
-
 Met5-Arg6-enkephalin
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 hydrolysis of benzoyl-Ala-Lys Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9 pH 5.5: 25% of maximal activity, pH 9.0: about 60% of maximal activity Homo sapiens

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
 1 h, room temperature, 33% loss of activity Homo sapiens
4.3
-
 1 h, room temperature, 17% loss of activity Homo sapiens
4.5 5 1 h, room temperature, stable Homo sapiens