Literature summary for 3.4.17.11 extracted from

Sadeghian, I.; Khalvati, B.; Ghasemi, Y.; Hemmati, S.
TAT-mediated intracellular delivery of carboxypeptidase G2 protects against methotrexate-induced cell death in HepG2 cells (2018), Toxicol. Appl. Pharmacol., 346, 9-18 .

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Application

Application	Comment	Organism
medicine	side effects of methotrexate especially hepatotoxicity limits clinical applications of this anticancer agent. Carboxypeptidase G2 fused to the transactivator transduction domain (TAT) is administrated for the treatment of elevated plasma concentrations of methotrexate. TAT-CPG2 significantly prevents methotrexate-induced oxidative stress by decreasing the formation of reactive oxygen species (ROS) and increasing the content of glutathione (GSH) and catalase activity. Both native and denatured TAT-carboxypeptidase G2 strongly protect HepG2 cells against methotrexate-induced oxidative stress and apoptosis. Intracellular delivery of carboxypeptidase G2 might provide a new therapeutic strategy for protecting against methotrexate mediated cytotoxicity	Pseudomonas sp. RS-16

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas sp. RS-16	P06621	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
methotrexate + H2O	-	Pseudomonas sp. RS-16	4-[(2,4-diamino-6-(pteridinyl)methyl)-methylamino]-benzoic acid + L-glutamate	-	?

Synonyms

Synonyms	Comment	Organism
carboxypeptidase G2	-	Pseudomonas sp. RS-16
CPG2	-	Pseudomonas sp. RS-16

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Release 2023.1 (January 2023)