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Literature summary for 3.4.17.11 extracted from

  • Goldman, P.; Levy, C.C.
    Carboxypeptidase G: purification and properties (1967), Proc. Natl. Acad. Sci. USA, 58, 1299-1306.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.17.11

General Stability

General Stability Organism
activity can increase up to 30% if the enzyme at all stages of purification is stored at -20°C Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
Ac-Glu inhibition of methotrexate hydrolysis Pseudomonas sp.
Ala-Glu inhibition of methotrexate hydrolysis Pseudomonas sp.
Benzoyl-Glu inhibition of methotrexate hydrolysis Pseudomonas sp.
Benzyloxycarbonyl-Glu inhibition of methotrexate hydrolysis Pseudomonas sp.
Benzyloxycarbonyl-Gly-Glu inhibition of methotrexate hydrolysis Pseudomonas sp.
Glu-Glu inhibition of methotrexate hydrolysis Pseudomonas sp.
Gly-Glu inhibition of methotrexate hydrolysis Pseudomonas sp.
His-Glu inhibition of methotrexate hydrolysis Pseudomonas sp.
Ile-Glu inhibition of methotrexate hydrolysis Pseudomonas sp.
Phe-Glu inhibition of methotrexate hydrolysis Pseudomonas sp.
Tyr-Glu inhibition of methotrexate hydrolysis Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ required Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Pseudomonas sp.

Storage Stability

Storage Stability Organism
-196°C, stable for several months Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
folic acid + H2O
-
 Pseudomonas sp. pteroic acid + glutamic acid
-
 ?
methotrexate + H2O
-
 Pseudomonas sp. 4-amino-4-deoxy-N10-methylpteroic acid + L-glutamate
-
 ?
additional information rapid hydrolysis occurs in linkages of the form RCO-Glu and RCO-Gln, whereas those of the form RCO-D-Glu (D-Glu-D-Glu and Z-D-Glu) are not attacked Pseudomonas sp. ?
-
 ?
additional information no hydrolysis of glutamate or glutamine lacking a free carboxyl alpha-carboxyl Pseudomonas sp. ?
-
 ?