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Literature summary for 3.4.17.10 extracted from

  • Hosaka, M.; Watanabe, T.; Sakai, Y.; Kato, T.; Takeuchi, T.
    Interaction between secretogranin III and carboxypeptidase E facilitates prohormone sorting within secretory granules (2005), J. Cell Sci., 118, 4785-4795.
    View publication on PubMed
Search for more literature for 3.4.17.10

Cloned(Commentary)

Cloned (Comment) Organism
expression as GST-fusion protein in Escherichia coli strain BL21(DE3) Mus musculus

Protein Variants

Protein Variants Comment Organism
additional information enzyme-defective CPEfat mice show elevated levels of secretogranin III and chromogranin A in the pituitary compensating for the lacking CPE sorting function, overview Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
additional information excessive secretogranin III leads to a decrease in CPE-bound proopiomelanocortin-derived intermediate molecules Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Mus musculus 16020
-
secretory granule the enzyme binds specifically to cholesterol-rich secretory granule membranes competing with secretogranin III, excessive secretogranin III leads to a decrease in CPE-bound proopiomelanocortin-derived intermediate molecules Mus musculus 30141
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ enhances the binding of CPE to secretogranin III Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Mus musculus interaction between secretogranin III and carboxypeptidase E facilitates prohormone sorting within secretory granules, both proteins form the separate functional sorting complex by anchoring in the cholesterol-rich membranes of the secretory granules, overview, secretogranin III can funtionally compensate for CPEin enzyme-defective mutant mice ?
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-fusion protein from Escherichia coli strain BL21(DE3) by glutathione affinity, native pro- or mature enzyme from AtT20 cells, best at pH 5.5 in presence of Ca2+ by GST-secretogranin III affinity Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
AtT-20 cell pituitary corticotrope-derived cells Mus musculus
-
endocrine gland
-
 Mus musculus
-
pituitary gland
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dansyl-Gly-Lys + H2O
-
 Mus musculus dansyl-Gly + Lys
-
 ?
additional information interaction between secretogranin III and carboxypeptidase E facilitates prohormone sorting within secretory granules, both proteins form the separate functional sorting complex by anchoring in the cholesterol-rich membranes of the secretory granules, overview, secretogranin III can funtionally compensate for CPEin enzyme-defective mutant mice Mus musculus ?
-
 ?
additional information the enzyme binds to secretogranin III Mus musculus ?
-
 ?

Synonyms

Synonyms Comment Organism
CPE
-
 Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 assay at Mus musculus