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Literature summary for 3.4.17.1 extracted from

  • Sui, Y.P.; Liu, X.B.; Chai, L.Q.; Wang, J.X.; Zhao, X.F.
    Characterization and influences of classical insect hormones on the expression profiles of a molting carboxypeptidase A from the cotton bollworm (Helicoverpa armigera) (2009), Insect Mol. Biol., 18, 353-363.
    View publication on PubMed
Search for more literature for 3.4.17.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) cells Helicoverpa armigera

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53600
-
 calculated from amino acid sequence Helicoverpa armigera
54000
-
 SDS-PAGE Helicoverpa armigera

Organism

Organism UniProt Comment Textmining
Helicoverpa armigera
-
 cotton bollworm
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme is purified by His-Bind resin column chromatography, endogenous enzyme is purified by CNBr-activated Sepharose column chromatography Helicoverpa armigera

Renatured (Commentary)

Renatured (Comment) Organism
the purified protein is refolded in the deionized water for 16 h at 4°C Helicoverpa armigera

Source Tissue

Source Tissue Comment Organism Textmining
fat body
-
 Helicoverpa armigera
-
hemocyte
-
 Helicoverpa armigera
-
integument integument from the 5th instar larvae Helicoverpa armigera
-
midgut
-
 Helicoverpa armigera
-
molting fluid molting fluid in the molting- and prepupal-stage larvae Helicoverpa armigera
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-(3-[2-furyl]acryloyl)-L-Phe-L-Phe + H2O
-
 Helicoverpa armigera N-(3-[2-furyl]acryloyl)-L-Phe + L-Phe
-
 ?

Synonyms

Synonyms Comment Organism
mCPA
-
 Helicoverpa armigera
molting carboxypeptidase A
-
 Helicoverpa armigera

pI Value

Organism Comment pI Value Maximum pI Value
Helicoverpa armigera calculated from amino acid sequence
-
 6.2

Expression

Organism Comment Expression
Helicoverpa armigera the expression of CPA can be up-regulated by 20-hydroxyecdysone. In the integument, the transcription level of the gene reaches its peak at the 5th instar molting stage and the 6th instar prepupal stage, respectively up

General Information

General Information Comment Organism
physiological function CPA participates in the apolysis of the integument during larval molting and metamorphosis Helicoverpa armigera