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Literature summary for 3.4.17.1 extracted from
- Two metallocarboxypeptidases from the protozoan Trypanosoma cruzi belong to the M32 family, found so far only in prokaryotes (2007), Biochem. J., 401, 399-410.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | stimulates, at 1 mM 168% activity relative to control | Trypanosoma cruzi |  |
Application
| Application | Comment | Organism |
|---|---|---|
| drug development | potential target for drug development | Trypanosoma cruzi |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli XL1 Blue cells as catalytically active poly-His-tagged recombinant enzyme. GST-fusion proteins expressed in Escherichia coli BL21 Codon Plus (DE3) cells | Trypanosoma cruzi |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 10 mM strongly inhibits | Trypanosoma cruzi | |
| additional information | PMSF at 2 mM, E-64 at 0.001 mM and Mn2+, Ni2+, Ca2+ and Mg2+ at 1 mM have no effect on activity | Trypanosoma cruzi | |
| o-phenanthroline | 1 mM strongly inhibits | Trypanosoma cruzi | |
| Zn2+ | at 0.1 mM 27.5% activity relative to control | Trypanosoma cruzi | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.024 | - |
N-(3-[2-furyl]acryloyl)-Phe-Phe | in the absence of Co2+ | Trypanosoma cruzi | |
| 0.034 | - |
N-(3-[2-furyl]acryloyl)-Phe-Phe | in the presence of Co2+ | Trypanosoma cruzi | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Trypanosoma cruzi | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58000 | - |
x * 58300, sequence analysis, x * 58000, polyclonal anti-MCP-2 peptide serum | Trypanosoma cruzi |
| 58300 | - |
x * 58300, sequence analysis, x * 58000, polyclonal anti-MCP-2 peptide serum | Trypanosoma cruzi |
| 132000 | - |
gel filtration | Trypanosoma cruzi |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trypanosoma cruzi | Q6ZXB9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purified in one step using Co-NTA resin | Trypanosoma cruzi |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| epimastigote | - |
Trypanosoma cruzi | - |
| metacyclic form | - |
Trypanosoma cruzi | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-(3-[2-furyl]acryloyl)-Phe-Phe | - |
Trypanosoma cruzi | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 58300, sequence analysis, x * 58000, polyclonal anti-MCP-2 peptide serum | Trypanosoma cruzi |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MCP-2 | - |
Trypanosoma cruzi |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
residual activity after 5 min of incubation at 50°C is 27.5%, the enzyme is not thermostable | Trypanosoma cruzi |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 7.6 | - |
N-(3-[2-furyl]acryloyl)-Phe-Phe | in the absence of Co2+ | Trypanosoma cruzi | |
| 11 | - |
N-(3-[2-furyl]acryloyl)-Phe-Phe | in the presence of Co2+ | Trypanosoma cruzi | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
- |
Trypanosoma cruzi |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | 8 | - |
Trypanosoma cruzi |
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