Cloned (Comment) | Organism |
---|---|
expression of CPA4 in Pichia pastoris, scecretion of the recombinant enzyme to the culture medium | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
purified recombinant CPA4 in complex with latexin, hanging drop vapour diffusion method, 0.001 ml of 7 mg/ml protein solution is mixed with 0.001 ml reservoir solution containing 40% 2-methyl-2,4-pentanediol, 0.1 M [bis(2-hydroxyethyl)amino]tris(hydroxymethyl)methane, pH 6.5, and with 0.002 ml 40% acetone, 20°C, X-ray diffraction structure determination and analysis at 1.6 A resolution | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
latexin | cloned from human brain and expressed in Escherichia coli, CPA4-latexin complex structure determination and analysis | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-(methoxyphenyl-azoformyl)-Phe-OH + H2O | - |
Homo sapiens | ? | - |
? | |
N-[3-(2-furyl)acryloyl]-Phe-Phe + H2O | - |
Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
carboxypeptidase A4 | - |
Homo sapiens |
CPA4 | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000003 | - |
latexin | pH 7.5, recombinant CPA4 | Homo sapiens |