Literature summary for 3.4.17.1 extracted from

Bradley, G.; Naude, R.J.; Muramoto, K.; Yamauchi, F.; Oeloefsen, W.
Ostrich (Struthio camelus) carboxypeptidase A: purification, kinetic properties and characterization of the pancreatic enzyme (1994), Int. J. Biochem., 26, 555-564.

Search for more literature for 3.4.17.1

Inhibitors

Inhibitors	Comment	Organism	Structure
3-Phenylpropionic acid	Ki: 0.482 mM	Struthio camelus
aminobenzylsuccinic acid	Ki: 0.039 mM	Struthio camelus
D-Phe	Ki: 0.074 mM	Struthio camelus
D-Trp	Ki: 0.014 mM	Struthio camelus
potato carboxypeptidase inhibitor	Ki: 107 nM	Struthio camelus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.15	-	hippuryl-DL-phenyllactate	-	Struthio camelus
0.16	-	carbobenzoxy-Gly-L-Leu	-	Struthio camelus
0.41	-	hippuryl-L-Phe	-	Struthio camelus
0.71	-	carbobenzoxy-Gly-L-Phe	-	Struthio camelus
0.74	-	Carbobenzoxy-Gly-Gly-Leu	-	Struthio camelus
1.34	-	Carbobenzoxy-Gly-Gly-Phe	-	Struthio camelus
2.11	-	carbobenzoxy-Gly-Gly-Val	-	Struthio camelus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	-	Struthio camelus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
34400	-	SDS-PAGE, type beta	Struthio camelus
34600	-	SDS-PAGE, type gamma	Struthio camelus

Organism

Organism	UniProt	Comment	Textmining
Struthio camelus	-	beta and gamma form	-

Purification (Commentary)

Purification (Comment)	Organism
-	Struthio camelus

Source Tissue

Source Tissue	Comment	Organism	Textmining
pancreas	-	Struthio camelus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
17.24	-	-	Struthio camelus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
carbobenzoxy-Gly-Gly-Leu + H2O	-	Struthio camelus	carbobenzoxy-Gly-Gly + Leu	-	ir
carbobenzoxy-Gly-Gly-Phe + H2O	-	Struthio camelus	carbobenzoxy-Gly-Gly + Phe	-	ir
carbobenzoxy-Gly-Gly-Val + H2O	-	Struthio camelus	carbobenzoxy-Gly-Gly + Val	-	ir
carbobenzoxy-Gly-L-Leu + H2O	-	Struthio camelus	carbobenzoxy-Gly + L-Leu	-	ir
carbobenzoxy-Gly-L-Phe + H2O	-	Struthio camelus	carbobenzoxy-Gly + L-Phe	-	ir
hippuryl-DL-phenyllactate + H2O	-	Struthio camelus	hippuric acid + DL-phenyllactate	-	ir
hippuryl-L-Phe + H2O	-	Struthio camelus	hippuric acid + L-Phe	-	ir

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Struthio camelus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
40	-	below	Struthio camelus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.081	-	hippuryl-DL-phenyllactate	-	Struthio camelus
1.28	-	Carbobenzoxy-Gly-Leu	-	Struthio camelus
5.02	-	Carbobenzoxy-Gly-Gly-Leu	-	Struthio camelus
5.85	-	Carbobenzoxy-Gly-Phe	-	Struthio camelus
5.98	-	hippuryl-Phe	-	Struthio camelus
6.13	-	carbobenzoxy-Gly-Gly-Val	-	Struthio camelus
11.9	-	Carbobenzoxy-Gly-Gly-Phe	-	Struthio camelus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Struthio camelus
8	8.5	-	Struthio camelus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.000107	-	potato carboxypeptidase inhibitor	-	Struthio camelus
0.014	-	D-Trp	-	Struthio camelus
0.039	-	aminobenzylsuccinic acid	-	Struthio camelus
0.074	-	D-Phe	-	Struthio camelus
0.482	-	3-Phenylpropionic acid	-	Struthio camelus

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Release 2023.1 (January 2023)