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Literature summary for 3.4.17.1 extracted from
- Purification and crystallization of human carboxypeptidase A (1976), Biochemistry, 15, 2501-2508.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Homo sapiens |
General Stability
| General Stability | Organism |
|---|---|
| effect of ionic strength: marked decrease of activity above 150 mM NaCl | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | 80% inhibition at 0.1 mM | Homo sapiens |  |
| Benzylsuccinic acid | Ki: 0.0016 | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Homo sapiens | |
| 0.1 | - |
benzoyl-Gly-Gly-L-alpha-hydroxy-beta-phenyllactate | - |
Homo sapiens | |
| 0.2 | - |
benzoyl-Gly-L-alpha-hydroxy-beta-phenyllactate | - |
Homo sapiens | |
| 0.23 | - |
benzoyl-Gly-L-Phe | at 0.1-0.4 mM substrate | Homo sapiens | |
| 3 | - |
carbobenzoxy-Gly-L-Phe | at 1-4 mM substrate | Homo sapiens | |
| 10 | - |
benzoyl-Gly-L-Phe | at 1-10 mM substrate | Homo sapiens | |
| 13.3 | - |
carbobenzoxy-Gly-L-Phe | at 4-40 mM substrate | Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | efficiency of metals in decreasing order: Zn, Co, Ni, Mn, Cd | Homo sapiens | |
| Cd2+ | substitution for native Zn, activates apoenzyme | Homo sapiens | |
| Co2+ | substitution for native Zn | Homo sapiens | |
| Co2+ | efficiency of metals in decreasing order: Zn, Co, Ni, Mn, Cd | Homo sapiens | |
| Mn2+ | substitution for native Zn | Homo sapiens | |
| Mn2+ | efficiency of metals in decreasing order: Zn, Co, Ni, Mn, Cd | Homo sapiens | |
| Ni2+ | substitution for native Zn | Homo sapiens | |
| Ni2+ | efficiency of metals in decreasing order: Zn, Co, Ni, Mn, Cd | Homo sapiens | |
| Zn2+ | metalloenzyme | Homo sapiens | |
| Zn2+ | 1 mol Zn per mol of enzyme | Homo sapiens | |
| Zn2+ | 1,01 mol Zn per mol enzyme | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
gel filtration | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pancreas | activated pancreatic juice | Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzoyl-Gly-Gly-L-alpha-hydroxy-beta-phenyllactate + H2O | - |
Homo sapiens | benzoyl-Gly-Gly-L-alpha-hydroxy-beta-phenyllactate + ? | - |
ir | |
| benzoyl-Gly-Gly-L-Phe + H2O | - |
Homo sapiens | benzoyl-Gly-Gly + L-Phe | - |
ir | |
| benzoyl-Gly-L-alpha-hydroxy-beta-phenyllactate + H2O | - |
Homo sapiens | ? | - |
? | |
| benzoyl-Gly-L-Phe + H2O | - |
Homo sapiens | benzoyl-Gly + L-Phe | - |
ir | |
| carbobenzoxy-Gly-L-Phe + H2O | - |
Homo sapiens | carbobenzoxy-Gly + L-Phe | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | amino acid composition described | Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
assay at | Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
50 min, 90% loss of activity | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 11.7 | - |
benzoyl-Gly-L-Phe | at 0.1-0.4 mM substrate | Homo sapiens | |
| 13.3 | - |
benzoyl-Gly-Gly-L-Phe | - |
Homo sapiens | |
| 48.3 | - |
carbobenzoxy-Gly-L-Phe | at 1-4 mM substrate | Homo sapiens | |
| 66.7 | - |
benzoyl-Gly-L-Phe | at 1-10 mM substrate | Homo sapiens | |
| 112 | - |
carbobenzoxy-Gly-L-Phe | at 4-40 mM substrate | Homo sapiens | |
| 505 | - |
benzoyl-Gly-L-alpha-hydroxy-beta-phenyllactate | - |
Homo sapiens | |
| 767 | - |
benzoyl-Gly-Gly-L-alpha-hydroxy-beta-phenyllactate | - |
Homo sapiens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Homo sapiens |
| 7.5 | 8 | - |
Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9.5 | pH 6.0: about 25% of maximum activity, pH 9.5: about 70% of maximum activity | Homo sapiens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
completely inactive at pH 5 | Homo sapiens |
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