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Literature summary for 3.4.16.6 extracted from

  • Morita, H.; Tomita, S.; Maeda, H.; Okamoto, A.; Yamagata, Y.; Kusumoto, K.; Amano, H.; Ishida, H.; Takeuchi, M.
    Serine-type carboxypeptidase KexA of Aspergillus oryzae has broader substrate specificity than Saccharomyces cerevisiae Kex1 and is required for normal hyphal growth and conidiation (2012), Appl. Environ. Microbiol., 78, 8154-8157.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
a truncated form of KexA (amino acid residues 1 to 526 of KexA), which lack the potential transmembrane region and the succeeding C-terminal sequence (amino acid residues 527 to 625 of KexA) is expressed by using a protein expression system with the pIECS3 vector and Aspergillus nidulans Aspergillus oryzae

Inhibitors

Inhibitors Comment Organism Structure
EDTA 14% inhibition Aspergillus oryzae
monoiodo acetic acid 90% inhibition Aspergillus oryzae
PMSF 68% inhibition Aspergillus oryzae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58820
-
 truncated form, calculated from cDNA Aspergillus oryzae

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using anion-exchange chromatography and gel filtration Aspergillus oryzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
angiotensin 1 + H2O truncated KexA releases amino acid residues from the C terminus of angiotensin I Aspergillus oryzae ?
-
 ?
benzoyl-Gly-Arg + H2O enzyme shows very low activity Aspergillus oryzae benzoyl-Gly + L-arginine
-
 ?
benzyloxycarbonyl-Gly-Lys + H2O enzyme shows very low activity Aspergillus oryzae benzyloxycarbonyl-Gly + L-lysine
-
 ?
benzyloxycarbonyl-Phe-Leu + H2O enzyme shows very low activity Aspergillus oryzae benzyloxycarbonyl-Phe + L-leucine
-
 ?
benzyloxycarbonyl-Phe-Tyr-Leu + H2O
-
 Aspergillus oryzae benzyloxycarbonyl-Phe-Tyr + L-leucine
-
 ?
benzyloxycarbonyl-Tyr-Leu + H2O enzyme shows very low activity Aspergillus oryzae benzyloxycarbonyl-Tyr + L-leucine
-
 ?
bradykinin I + H2O truncated KexA releases amino acid residues from the C terminus of bradykinin I Aspergillus oryzae ?
-
 ?

Synonyms

Synonyms Comment Organism
KexA
-
 Aspergillus oryzae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 maximum temperature at which the enzyme has residual activity of over 60% relative to maximum activity Aspergillus oryzae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Aspergillus oryzae

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 8
-
 Aspergillus oryzae

General Information

General Information Comment Organism
malfunction kexA deletion strain shows slightly lower growth speed. Deletion strain exhibits scarcely branched hyphae, whereas an kexA-overexpressing strain exhibits a hyperbranching phenotype Aspergillus oryzae