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Literature summary for 3.4.16.6 extracted from

  • Latchinian-Sadek, L.; Thomas, D.Y.
    Expression, purification, and characterization of the yeast KEX1 gene product, a polypeptide precursor processing carboxypeptidase (1993), J. Biol. Chem., 268, 534-540.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
1-chloro-3-tosylamido-7-amino-2-heptanone
-
Saccharomyces cerevisiae
alpha-Factor KR
-
Saccharomyces cerevisiae
Enkephalin heptapeptides
-
Saccharomyces cerevisiae
Guanidinoethylmercaptosuccinic acid
-
Saccharomyces cerevisiae
Hg2+
-
Saccharomyces cerevisiae
leupeptin
-
Saccharomyces cerevisiae
phenylmethylsulfonyl fluoride
-
Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.284
-
Benzoyl-Phe-Ala-Arg
-
Saccharomyces cerevisiae
0.516
-
furylacryloyl-Ala-Arg
-
Saccharomyces cerevisiae
0.962
-
furylacryloyl-Ala-Lys
-
Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates at low concentrations Saccharomyces cerevisiae
Mg2+ activates at low concentrations Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Saccharomyces cerevisiae plays a role in polypeptide processing in yeast ?
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
KEX1 gene from Saccharomyces cerevisiae expressed using the baculovirus/insect cell system
-

Purification (Commentary)

Purification (Comment) Organism
KEX1 gene from Saccharomyces cerevisiae expressed using the baculovirus/insect cell system Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
22.5
-
-
Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-factor-Lys-Arg + H2O maturation takes place in sequential manner Saccharomyces cerevisiae mature active alpha-factor + Lys + Arg
-
?
benzoyl-Phe-Ala-Arg + H2O
-
Saccharomyces cerevisiae benzoyl-Phe-Ala + Arg
-
?
furylacryloyl-Ala-Arg + H2O
-
Saccharomyces cerevisiae furylacryloyl-Ala + Arg
-
?
furylacryloyl-Ala-Lys + H2O
-
Saccharomyces cerevisiae furylacryloyl-Ala + Lys
-
?
additional information plays a role in polypeptide processing in yeast Saccharomyces cerevisiae ?
-
?
[Leu5]enkephalin-Arg6 + H2O
-
Saccharomyces cerevisiae ?
-
?
[Leu5]enkephalin-Lys6 + H2O
-
Saccharomyces cerevisiae ?
-
?
[Met5]enkephalin-Arg6 + H2O
-
Saccharomyces cerevisiae ?
-
?
[Met5]enkephalin-Lys6 + H2O
-
Saccharomyces cerevisiae ?
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Saccharomyces cerevisiae

pI Value

Organism Comment pI Value Maximum pI Value
Saccharomyces cerevisiae pI-value below pH 3.0
-
additional information