Literature summary for 3.4.16.5 extracted from

Kinsho, T.; Ueno, H.; Hayashi, R.; Hashizume, C.; Kimura, K.
Sub-zero temperature inactivation of carboxypeptidase Y under high hydrostatic pressure (2002), Eur. J. Biochem., 269, 4666-4674.

General Stability

General Stability	Organism
when the enzyme is treated at pressures higher than 300 mPa and temperatures lower than -5°C, it undergoes an irreversible inactivation in which nearly 50% of the alpha-helical structure is lost as judged by circular dichroism spectral analysis. When the applied pressure is limited to below 200 mPa, the cold inactivation process appears to be reversible. In the presence of reducing agent, this reversible phenomenon, observed at below 200 mPa, diminishes to give an inactive enzyme	Saccharomyces cerevisiae

Organism

when the enzyme is treated at pressures higher than 300 mPa and temperatures lower than -5°C, it undergoes an irreversible inactivation in which nearly 50% of the alpha-helical structure is lost as judged by circular dichroism spectral analysis. When the applied pressure is limited to below 200 mPa, the cold inactivation process appears to be reversible. In the presence of reducing agent, this reversible phenomenon, observed at below 200 mPa, diminishes to give an inactive enzyme

Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	-	-

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
additional information	-	when the enzyme is treated at pressures higher than 300 mPa and temperatures lower than -5°C, it undergoes an irreversible inactivation in which nearly 50% of the alpha-helical structure is lost as judged by circular dichroism spectral analysis. When the applied pressure is limited to below 200 mPa, the cold inactivation process appears to be reversible. In the presence of reducing agent, this reversible phenomenon, observed at below 200 mPa, diminishes to give an inactive enzyme	Saccharomyces cerevisiae
-30	20	30 min, 0.1 mPa, nearly full activity is maintained	Saccharomyces cerevisiae
10	-	400 mPa, 10 min, 50% loss of activity	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)