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Literature summary for 3.4.16.5 extracted from

  • Spr÷ssler, B.; Heilmann, H.D.; Grampp, E.; Uhlig, H.
    Properties of carboxypeptidase C isolated from orange leaves (1971), Hoppe-Seyler's Z. Physiol. Chem., 352, 1524-1530.
    View publication on PubMed
Search for more literature for 3.4.16.5

General Stability

General Stability Organism
citrus peel enzyme is inactivated upon lyophilization Citrus sp.
orange leaf enzyme is stable to lyophilization Citrus sp.

Inhibitors

Inhibitors Comment Organism Structure
2-propanol above 5% Citrus sp.
diisopropyl fluorophosphate peel enzyme inhibited, leaf enzyme not Citrus sp.
ethanol above 5% Citrus sp.
additional information not inhibition by EDTA Citrus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no activation by metal ions Citrus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
126000
-
 enzyme from peel, gel filtration Citrus sp.
175000
-
 enzyme from leaf, gel filtration Citrus sp.

Organism

Organism UniProt Comment Textmining
Citrus sp.
-
-
-
Citrus sp.
-
 orange
-

Purification (Commentary)

Purification (Comment) Organism
-
 Citrus sp.

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Citrus sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-Leu-Phe + H2O
-
 Citrus sp. benzyloxycarbonyl-Leu + Phe
-
 ?
additional information
-
 Citrus sp. ?
-
 ?
additional information removes C-terminal aromatic, neutral acid and basic amino acids, proline bonds are hydrolyzed on both the amino and carboxyl side, glycine is attacked slowly Citrus sp. ?
-
 ?
additional information owing to its low specificity very suitable for sequence analysis Citrus sp. ?
-
 ?
additional information no hydrolysis of benzyloxycarbonyl-Gly-Pro Citrus sp. ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 20 min incubation, benzyloxycarbonyl-Leu-Phe Citrus sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30
-
 30°C: about 65% of activity maximum, 60°C: about 70% of activity maximum Citrus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3
-
-
 Citrus sp.

pH Range

pH Minimum pH Maximum Comment Organism
4.5 6 pH 4.5: about 70% of activity maximum, pH 6: about 55% of activity maximum Citrus sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 6 orange leaf enzyme is unstable below pH 4 and above pH 6 Citrus sp.
5
-
 most stable at room temperature Citrus sp.