KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
3-carboxyphenyl (2R)-N-(phenylacetyl)-2-methoxyglycinate | pH 7.5, 25°C | Actinomadura sp. | |
0.36 | - |
3-carboxyphenyl (2R)-N-(phenylacetyl)-2-hydroxyglycinate | pH 7.5, 25°C | Streptomyces sp. | |
0.45 | - |
3-carboxyphenyl (2R)-N-(phenylacetyl)-2-methoxyglycinate | pH 7.5, 25°C | Streptomyces sp. | |
4.3 | - |
3-carboxyphenyl (2R)-N-(phenylacetyl)-2-hydroxyglycinate | pH 7.5, 25°C | Actinomadura sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Actinomadura sp. | P39045 | - |
- |
Streptomyces sp. | P15555 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-carboxyphenyl (2R)-N-(phenylacetyl)-2-hydroxyglycinate + H2O | no reaction with (S)-enantiomer | Streptomyces sp. | (2R)-N-(phenylacetyl)-2-hydroxyglycine + 3-hydroxybenzoate | substrate for both beta-lactamases and D,D-peptidases. Both prefer the same enantiomer, the (R)-form | ? | |
3-carboxyphenyl (2R)-N-(phenylacetyl)-2-hydroxyglycinate + H2O | no reaction with (S)-enantiomer | Actinomadura sp. | (2R)-N-(phenylacetyl)-2-hydroxyglycine + 3-hydroxybenzoate | substrate for both beta-lactamases and D,D-peptidases. Both prefer the same enantiomer, the (R)-form | ? | |
3-carboxyphenyl (2R)-N-(phenylacetyl)-2-methoxyglycinate | no reaction with (S)-enantiomer | Streptomyces sp. | (2R)-N-(phenylacetyl)-2-methoxyglycine + 3-hydroxybenzoate | substrate for both beta-lactamases and D,D-peptidases. Both prefer the same enantiomer, the (R)-form | ? | |
3-carboxyphenyl (2R)-N-(phenylacetyl)-2-methoxyglycinate | no reaction with (S)-enantiomer | Actinomadura sp. | (2R)-N-(phenylacetyl)-2-methoxyglycine + 3-hydroxybenzoate | substrate for both beta-lactamases and D,D-peptidases. Both prefer the same enantiomer, the (R)-form | ? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4 | - |
3-carboxyphenyl (2R)-N-(phenylacetyl)-2-methoxyglycinate | pH 7.5, 25°C | Streptomyces sp. | |
6.9 | - |
3-carboxyphenyl (2R)-N-(phenylacetyl)-2-hydroxyglycinate | pH 7.5, 25°C | Streptomyces sp. | |
10.4 | - |
3-carboxyphenyl (2R)-N-(phenylacetyl)-2-hydroxyglycinate | pH 7.5, 25°C | Actinomadura sp. | |
114 | - |
3-carboxyphenyl (2R)-N-(phenylacetyl)-2-methoxyglycinate | pH 7.5, 25°C | Actinomadura sp. |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
19 | - |
3-carboxyphenyl (2R)-N-(phenylacetyl)-2-hydroxyglycinate | pH 7.5, 25°C | Streptomyces sp. | |
24 | - |
3-carboxyphenyl (2R)-N-(phenylacetyl)-2-hydroxyglycinate | pH 7.5, 25°C | Actinomadura sp. | |
98 | - |
3-carboxyphenyl (2R)-N-(phenylacetyl)-2-methoxyglycinate | pH 7.5, 25°C | Streptomyces sp. | |
570 | - |
3-carboxyphenyl (2R)-N-(phenylacetyl)-2-methoxyglycinate | pH 7.5, 25°C | Actinomadura sp. |