Literature summary for 3.4.16.4 extracted from

Kumar, I.; Pratt, R.F.
Transpeptidation reactions of a specific substrate catalyzed by the Streptomyces R61 DD-peptidase: Characterization of a chromogenic substrate and acyl acceptor design (2005), Biochemistry, 44, 9971 -9979.

Search for more literature for 3.4.16.4

Activating Compound

Activating Compound	Comment	Organism	Structure
aminomalon-(N-ethyl)amide	dual function acceptor, which is particularly effective	Streptomyces sp. R61
aminomalon-(N-phenethyl)amide	dual function acceptor, which is particularly effective	Streptomyces sp. R61
D-phenylalanine	most effective D-amino acid and dipeptide acceptor, respectively	Streptomyces sp. R61
Gly-L-Phe	most effective D-amino acid and dipeptide acceptor, respectively	Streptomyces sp. R61
N-ethyl-D-asparagine	less effective acceptor	Streptomyces sp. R61

Inhibitors

Inhibitors	Comment	Organism	Structure
alpha-N-glycyl-epsilon-N-acetyl-L-lysine	inhibits hydrolysis of 3-(N-glycyl-L-cysteinyl)-propanoyl-D-alanyl-D-thiolactate	Streptomyces sp. R61
epsilon-N-glycyl-alpha-N-acetyl-L-alanyl-D-isoglutaminyl-L-lysine	inhibits hydrolysis of 3-(N-glycyl-L-cysteinyl)-propanoyl-D-alanyl-D-thiolactate	Streptomyces sp. R61
N-(glycyl)-D,L-alpha-aminocaprylic acid	inhibits hydrolysis of 3-(N-glycyl-L-cysteinyl)-propanoyl-D-alanyl-D-thiolactate	Streptomyces sp. R61

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	-	Streptomyces sp. R61
0.004	-	3-(N-glycyl-L-cysteinyl)-propanoyl-D-alanyl-D-thiolactate	-	Streptomyces sp. R61

Organism

Organism	UniProt	Comment	Textmining
Streptomyces sp. R61	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3-(N-glycyl-L-cysteinyl)-propanoyl-D-alanyl-D-thiolactate + H2O	very efficient substrate for R61 DD-peptidase	Streptomyces sp. R61	3-(N-glycyl-L-cysteinyl)-propanoyl-D-alanine + D-thiolactate	-	?

Synonyms

Synonyms	Comment	Organism
DD-peptidase	-	Streptomyces sp. R61
penicillin-binding protein	-	Streptomyces sp. R61

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
additional information	-	additional information	-	Streptomyces sp. R61
42	-	3-(N-glycyl-L-cysteinyl)-propanoyl-D-alanyl-D-thiolactate	-	Streptomyces sp. R61

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Release 2023.1 (January 2023)