Literature summary for 3.4.16.2 extracted from

Mallela, J.; Perkins, R.; Yang, J.; Pedigo, S.; Rimoldi, J.M.; Shariat-Madar, Z.
The functional importance of the N-terminal region of human prolylcarboxypeptidase (2008), Biochem. Biophys. Res. Commun., 374, 635-640.

Search for more literature for 3.4.16.2

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Schneider insect cells	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
DELTA317-496	an N-terminal truncated form, lacking 179 N-terminal residues of PRCP is constructed. The circular dichroism spectrum of the truncated mutant illustrates that it is structured with significant helical content as indicated by local minima at 220 and 208 nm. The main products of angiotensin III metabolized by the truncated mutant is angiotensin 2-7 plus phenylalanine as determined by LC-MS. Angiotensin I blockes the metabolism of angiotensin III by the truncated mutant. Km (mM) (Ala-Pro-4-nitroanilide) lower compared to wild-type	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
angiotensin I	inhibits the mutant DELTA317-496 in a dose-dependent manner	Homo sapiens
angiotensin III	inhibits the mutant DELTA317-496 in a dose-dependent manner	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.5	-	Ala-Pro-4-nitroanilide	mutant DELTA317-496	Homo sapiens
3.1	-	Ala-Pro-4-nitroanilide	wild-type	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
40000	-	mutant DELTA317-496	Homo sapiens
52000	-	predicted from cDNA	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Ala-Pro-4-nitroanilide + H2O	-	Homo sapiens	Ala-Pro + 4-nitroaniline	-	?
angiotensin III + H2O	mutant DELTA317-496 is able to carry out this reaction	Homo sapiens	angiotensin 2-7 + phenylalanine	-	?

Subunits

Subunits	Comment	Organism
?	x * 52000, calculated from cDNA	Homo sapiens

Synonyms

Synonyms	Comment	Organism
PRCP	-	Homo sapiens
prolylcarboxypeptidase	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5.8	-	assay at	Homo sapiens

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
1	-	-	Homo sapiens	angiotensin I

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Release 2023.1 (January 2023)