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Literature summary for 3.4.16.2 extracted from

  • Suga, K.; Ito, K.; Tsuru, D.; Yoshimoto, T.
    Prolylcarboxypeptidase (angiotensinase C): purification and characterization of the enzyme from Xanthomanas maltophilia (1995), Biosci. Biotechnol. Biochem., 59, 298-301.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
Stenotrophomonas maltophilia
iodoacetamide
-
Stenotrophomonas maltophilia
o-phenanthroline
-
Stenotrophomonas maltophilia
PCMB
-
Stenotrophomonas maltophilia
phenylmethylsulfonyl fluoride
-
Stenotrophomonas maltophilia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.26
-
N-benzyloxycarbonyl-L-Pro-Leu
-
Stenotrophomonas maltophilia
0.34
-
angiotensin II
-
Stenotrophomonas maltophilia

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
83000
-
4 * 83000, SDS-PAGE Stenotrophomonas maltophilia
330000
-
gel filtration Stenotrophomonas maltophilia

Organism

Organism UniProt Comment Textmining
Stenotrophomonas maltophilia
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Stenotrophomonas maltophilia

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Stenotrophomonas maltophilia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
angiotensin II + H2O
-
Stenotrophomonas maltophilia ?
-
?
N-benzyloxycarbonyl-L-Pro-Leu + H2O
-
Stenotrophomonas maltophilia N-benzyloxycarbonyl-L-Pro + L-Leu
-
?
N-benzyloxycarbonyl-Pro-Leu + H2O
-
Stenotrophomonas maltophilia N-benzyloxycarbonyl-Pro + Leu
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 83000, SDS-PAGE Stenotrophomonas maltophilia

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Stenotrophomonas maltophilia

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
pH 8.0, 60 min, 20% loss of activity Stenotrophomonas maltophilia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Stenotrophomonas maltophilia

pH Stability

pH Stability pH Stability Maximum Comment Organism
8 11 37°C, 60 min, 20% loss of activity Stenotrophomonas maltophilia