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Literature summary for 3.4.16.2 extracted from

  • Odya, C.E.; Marinkovic, D.V.; Hammon, K.J.; Stewart, T.A.; Erdös, E.G.
    Purification and properties of prolylcarboxypeptidase (angiotensinase C) from human kidney (1978), J. Biol. Chem., 253, 5927-5931.
    View publication on PubMed
Search for more literature for 3.4.16.2

Inhibitors

Inhibitors Comment Organism Structure
diisopropyl fluorophosphate
-
 Homo sapiens
pepstatin
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1
-
 angiotensin II
-
 Homo sapiens
2
-
 angiotensin III
-
 Homo sapiens
4.7
-
 N-benzyloxycarbonyl-L-Pro-L-Ala
-
 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 x * 45000 + x * 66500, SDS-PAGE in presence of 2-mercaptoethanol Homo sapiens
66500
-
 x * 45000 + x * 66500, SDS-PAGE in presence of 2-mercaptoethanol Homo sapiens
115000
-
 gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
angiotensin II + H2O about 35% of the activity with angiotensin III Homo sapiens ?
-
 ?
angiotensin III + H2O
-
 Homo sapiens ?
-
 ?
N-benzyloxycarbonyl-L-Pro-L-Ala + H2O
-
 Homo sapiens N-benzyloxycarbonyl-L-Pro + L-Ala
-
 ?
N-benzyloxycarbonyl-Pro-Ala + H2O 33% of the activity with angiotensin III Homo sapiens N-benzyloxycarbonyl-Pro + Ala
-
 ?
N-benzyloxycarbonyl-Pro-Leu + H2O 12% of the activity with angiotensin III Homo sapiens N-benzyloxycarbonyl-Pro + Leu
-
 ?
N-benzyloxycarbonyl-Pro-Phe + H2O 8% of the activity with angiotensin III Homo sapiens N-benzyloxycarbonyl-Pro + Phe
-
 ?
N-benzyloxycarbonyl-Pro-Ser + H2O 7% of the activity with angiotensin III Homo sapiens N-benzyloxycarbonyl-Pro + Ser
-
 ?
N-benzyloxycarbonyl-Pro-Val + H2O 28% of the activity with angiotensin III Homo sapiens N-benzyloxycarbonyl-Pro + Val
-
 ?
saralasin + H2O
-
 Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
? x * 45000 + x * 66500, SDS-PAGE in presence of 2-mercaptoethanol Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5 5.5
-
 Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
4.5 7 pH 4.5-5.0: optimal pH with N-benzyloxycarbonyl-Pro-Phe, angiotensin II or angiotensin III as substrate, pH 7: 9% of maximal activity with N-benzyloxycarbonyl-Pro-Phe, 20% of maximal activity with angiotensin II, 50% of maximal activity with angiotensin III Homo sapiens