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Literature summary for 3.4.15.5 extracted from
- Dipeptidyl carboxypeptidase from Escherichia coli (1976), Methods Enzymol., 45, 599-610.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
Lys4 | - |
Escherichia coli |  |
| 0.44 | - |
Ala4 | - |
Escherichia coli | |
| 0.61 | - |
Ala-Gly-Phe-Ala | - |
Escherichia coli | |
| 0.71 | - |
tert-butyloxycarbonyl-Ala-Ala-Ala | - |
Escherichia coli | |
| 1.27 | - |
Ala-Ala-Phe-Ala | - |
Escherichia coli | |
| 1.39 | - |
Gly-Ala-Phe-Ala | - |
Escherichia coli | |
| 6.06 | - |
Gly-Gly-Phe-Ala | - |
Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | activates | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 97000 | - |
E. coli B, gradient PAGE | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
B | - |
| Escherichia coli B / ATCC 11303 | - |
B | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, retains 70% of the original activity after 5 weeks | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Ala-Ala-Ala-Ala + H2O | - |
Escherichia coli | Ala-Ala + Ala-Ala | - |
? | |
| Ala-Ala-Ala-Ala + H2O | - |
Escherichia coli B / ATCC 11303 | Ala-Ala + Ala-Ala | - |
? | |
| Ala-Ala-Ala-Lys-Phe + H2O | - |
Escherichia coli | Ala-Ala-Ala + Lys-Phe | - |
? | |
| Ala-Ala-Phe-Ala + H2O | - |
Escherichia coli | Ala-Ala + Phe-Ala | - |
? | |
| Ala-Gly-Phe-Ala + H2O | - |
Escherichia coli | Ala-Gly + Phe-Ala | - |
? | |
| Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg + H2O | - |
Escherichia coli | ? | - |
? | |
| Benzyloxycarbonyl-Ala-Ala-Ala + H2O | - |
Escherichia coli | Benzyloxycarbonyl-Ala + Ala-Ala | - |
? | |
| Benzyloxycarbonyl-Ala-Ala-Ala + H2O | - |
Escherichia coli B / ATCC 11303 | Benzyloxycarbonyl-Ala + Ala-Ala | - |
? | |
| Dinitrophenyl-poly-(Pro-Gly-Pro) + H2O | - |
Escherichia coli | Gly-Pro + ? | - |
? | |
| Dinitrophenyl-poly-(Pro-Gly-Pro) + H2O | - |
Escherichia coli B / ATCC 11303 | Gly-Pro + ? | - |
? | |
| Gly-Ala-Phe-Ala + H2O | - |
Escherichia coli | Gly-Ala + Phe-Ala | - |
? | |
| Gly-Gly-Phe-Ala + H2O | - |
Escherichia coli | Gly-Gly + Phe-Ala | - |
? | |
| Lys-Ala-Ala-Lys-Ala-Ala + H2O | - |
Escherichia coli | Lys-Ala + Ala-Lys-Ala-Ala | - |
? | |
| Lys-Lys-Lys-Lys + H2O | - |
Escherichia coli | Lys-Lys + Lys-Lys | - |
? | |
| Lys-Lys-Lys-Lys + H2O | - |
Escherichia coli B / ATCC 11303 | Lys-Lys + Lys-Lys | - |
? | |
| additional information | the enzyme hydrolyzes the penultimate peptide bond of alpha-N-blocked tripeptides, free tetrapeptides and higher peptides | Escherichia coli | ? | - |
? | |
| additional information | the enzyme hydrolyzes the penultimate peptide bond of alpha-N-blocked tripeptides, free tetrapeptides and higher peptides | Escherichia coli B / ATCC 11303 | ? | - |
? | |
| tert-butyloxycarbonyl-Ala-Ala-Ala + H2O | - |
Escherichia coli | tert-butyloxycarbonyl-Ala + Ala-Ala | - |
? | |
| tert-Butyloxycarbonyl-Ala-Glu-Ala-Ala + H2O | - |
Escherichia coli | tert-Butyloxycarbonyl-Ala-Glu + Ala-Ala | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.2 | - |
- |
Escherichia coli |
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