Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cetyltrimethylammonium bromide | CTAB, activates at 0.1-1.0 mM | Struthio camelus australis | |
SDS | activates at 0.1 mM | Struthio camelus australis | |
Triton X-100 | activates at 0.01%, inhibits at 1.0-10.0% | Struthio camelus australis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | inhibitory at concentrations above 1 mM | Struthio camelus australis | |
captopril | - |
Struthio camelus australis | |
Co2+ | inhibitory at concentrations above 1 mM | Struthio camelus australis | |
EDTA | complete inhibition at 0.01 mM | Struthio camelus australis | |
Mg2+ | inhibitory at concentrations above 1 mM | Struthio camelus australis | |
additional information | PMSF has no effect on the ACE activity | Struthio camelus australis | |
o-phenanthroline | complete inhibition at 0.35 mM | Struthio camelus australis | |
trifluoroethanol | TFE, stabilizes ACE at low concentrations, while acts as a denaturant at higher concentration (20%). Secondary and tertiary structure and activity of ACE in the absence and presence of TFE are investigated using circular dichroism, fluorescence quenching, and UV-visible spectroscopy, respectively | Struthio camelus australis | |
Triton X-100 | activates at 0.01%, inhibits at 1.0-10.0% | Struthio camelus australis | |
Zn2+ | inhibitory at concentrations above 1 mM | Struthio camelus australis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
N-(3-[2-furyl]acryloyl)-L-phenylalanylglycylglycine | pH 7.5, 22°C | Struthio camelus australis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activates, but to a lesser extent than Zn2+ | Struthio camelus australis | |
Mn2+ | activates, but to a lesser extent than Zn2+ | Struthio camelus australis | |
Zn2+ | required, activates | Struthio camelus australis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
200000 | - |
gel filtration | Struthio camelus australis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
angiotensin I + H2O | Struthio camelus australis | - |
angiotensin II + His-Leu | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Struthio camelus australis | A0A093HJH8 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | ACE is highly glycosylated | Struthio camelus australis |
Purification (Comment) | Organism |
---|---|
native enzyme 447fold from lung by ammonium sulfate fractionation, gel filtration, and anion exchange chromatography, followed by ultrafiltration | Struthio camelus australis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
lung | - |
Struthio camelus australis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
147.52 | - |
purified native enzyme, pH 7.5, 22°C, substrate N-(3-[2-furyl]acryloyl)-L-phenylalanylglycylglycine | Struthio camelus australis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
angiotensin I + H2O | - |
Struthio camelus australis | angiotensin II + His-Leu | - |
? | |
N-(3-[2-furyl]acryloyl)-L-phenylalanylglycylglycine + H2O | - |
Struthio camelus australis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ACE | - |
Struthio camelus australis |
angiotensin I-converting enzyme | - |
Struthio camelus australis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Struthio camelus australis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
purified native enzyme, 30 min, completely stable up to | Struthio camelus australis |
55 | - |
purified native enzyme, 30 min, 90% activity reamining | Struthio camelus australis |
60 | - |
purified native enzyme, 30 min, inactivation | Struthio camelus australis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
987.3 | - |
N-(3-[2-furyl]acryloyl)-L-phenylalanylglycylglycine | pH 7.5, 22°C | Struthio camelus australis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Struthio camelus australis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 10.5 | activity range, inactive below pH 6.5 and above pH 10.5 | Struthio camelus australis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000166 | - |
captopril | pH 7.5, 22°C | Struthio camelus australis |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0000365 | - |
pH 7.5, 22°C | Struthio camelus australis | captopril |
General Information | Comment | Organism |
---|---|---|
additional information | secondary and tertiary structure and activity of ACE is investigated using circular dichroism, fluorescence quenching, and UV-visible spectroscopy, respectively | Struthio camelus australis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
123417 | - |
N-(3-[2-furyl]acryloyl)-L-phenylalanylglycylglycine | pH 7.5, 22°C | Struthio camelus australis |