Literature summary for 3.4.15.1 extracted from

Akif, M.; Georgiadis, D.; Mahajan, A.; Dive, V.; Sturrock, E.D.; Isaac, R.E.; Acharya, K.R.
High-resolution crystal structures of Drosophila melanogaster angiotensin-converting enzyme in complex with novel inhibitors and antihypertensive drugs (2010), J. Mol. Biol., 400, 502-517.

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Pichia pastoris	Drosophila melanogaster

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant free AnCE and in complex with six antihypertensive drugs, captopril, enalaprilat, lisinopril, ramiprilat, trandolaprilat, perindoprilat, lisW-S, RXPA380, and RXP407, hanging drop vapour diffusion method at 16 °C, 0.002 ml of protein solution containing 10 mg/ml native AnCE in 5 mM HEPES, pH 7.5, 0.1 mM PMSF, and 0.01 mM zinc acetate, are mixed with 0.002 ml of reservoir solution containing 0.1 M HEPES, pH 7.5, and 1.5 M sodium citrate, for enzyme complexes, the crystals are mixed with inhibitor solution, X-ray diffraction structue determination and analysis at 1.85-2.10 A resolution	Drosophila melanogaster

Crystallization (Comment)

Organism

purified recombinant free AnCE and in complex with six antihypertensive drugs, captopril, enalaprilat, lisinopril, ramiprilat, trandolaprilat, perindoprilat, lisW-S, RXPA380, and RXP407, hanging drop vapour diffusion method at 16 °C, 0.002 ml of protein solution containing 10 mg/ml native AnCE in 5 mM HEPES, pH 7.5, 0.1 mM PMSF, and 0.01 mM zinc acetate, are mixed with 0.002 ml of reservoir solution containing 0.1 M HEPES, pH 7.5, and 1.5 M sodium citrate, for enzyme complexes, the crystals are mixed with inhibitor solution, X-ray diffraction structue determination and analysis at 1.85-2.10 A resolution

Drosophila melanogaster

Inhibitors

Inhibitors	Comment	Organism
captopril	-	Drosophila melanogaster
enalaprilat	-	Drosophila melanogaster
lisinopril	-	Drosophila melanogaster
lisW-S	lisinopril-tryptophan S-enantiomer, a C-domain human sACE specific inhibitor and antihypertensive drug	Drosophila melanogaster
perindoprilat	-	Drosophila melanogaster
ramiprilat	-	Drosophila melanogaster
RXP407	i.e. Ac-Asp-L-Phe(PO2CH2)-L-Ala-Ala-NH2, a human sACE domain-specific phosphinic peptidyl inhibitor and antihypertensive drug	Drosophila melanogaster
RXPA380	i.e. (2S)-2-[([2-[(1R)-1-[((benzyloxy)carbonyl)amino]-2-(phenylethyl)(hydroxyl)-phosphinyl]cyclopentyl]carbonyl)amino]-3-(1H-indo-3-yl)-propionic acid (Cbz-PhePSI[P(O)(OH)CH]Pro-Trp-OH), a human sACE domain-specific phosphinic peptidyl inhibitor and antihypertensive drug	Drosophila melanogaster
trandolaprilat	-	Drosophila melanogaster

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Drosophila melanogaster	AnCE is a single domain protein with ACE activity, an ACE homologue	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Drosophila melanogaster	Q10714	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme from Pichia pastoris by hydrophobic interaction chromatography and gel filtration to homogeneity	Drosophila melanogaster

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	AnCE is a single domain protein with ACE activity, an ACE homologue	Drosophila melanogaster	?	-	?

Synonyms

Synonyms	Comment	Organism
ACE	-	Drosophila melanogaster
ANCE	-	Drosophila melanogaster
angiotensin I-converting enzyme	-	Drosophila melanogaster
angiotensin-converting enzyme	-	Drosophila melanogaster