Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.15.1 extracted from

  • Naqvi, N.; Liu, K.; Graham, R.M.; Husain, A.
    Molecular basis of exopeptidase activity in the C-terminal domain of human angiotensin I-converting enzyme: insights into the origins of its exopeptidase activity (2005), J. Biol. Chem., 280, 6669-6675.
    View publication on PubMed
Search for more literature for 3.4.15.1

Protein Variants

Protein Variants Comment Organism
K1087A no activity with angiotensin I, kcat/KM for [Phe9]angiotensin I is 654fold lower than wild-type value, kcat/KM for [Arg10]angiotensin I is 193fold lower than wild-type value, kcat/KM for [Phe9,Arg10]angiotensin I is 462fold lower than wild-type value Homo sapiens
R1098Q kcat/KM for angiotensin I is 7fold lower than wild-type value, kcat/KM for [Phe9]angiotensin I is 2.2fold higher than wild-type value, kcat/KM for [Arg10]angiotensin I is 23fold lower than wild-type value, kcat/KM for [Phe9,Arg10]angiotensin I is 1.4fold lower than wild-type value Homo sapiens
Y1096F kcat/KM for angiotensin I is 12fold lower than wild-type value, kcat/KM for [Phe9]angiotensin I is 8.9fold lower than wild-type value, kcat/KM for [Arg10]angiotensin I is 8.5fold lower than wild-type value, kcat/KM for [Phe9,Arg10]angiotensin I is 7fold lower than wild-type value Homo sapiens
Y1096F/K1087A no activity with angiotensin I, [Phe9]angiotensin I, [Arg10]angiotensin I, and [Phe9,Arg10]angiotensin I Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
 [Phe9,Arg10]angiotensin I 37°C, pH 7.5, wild-type enzyme Homo sapiens
0.014
-
 [Phe9,Arg10]angiotensin I 37°C, pH 7.5, mutant enzyme Y1096F Homo sapiens
0.02
-
 [Phe9,Arg10]angiotensin I 37°C, pH 7.5, mutant enzyme R1098Q Homo sapiens
0.025
-
 [Phe9,Arg10]angiotensin I 37°C, pH 7.5, mutant enzyme K1087A Homo sapiens
0.032
-
 [Phe9]angiotensin I 37°C, pH 7.5, wild-type enzyme Homo sapiens
0.042
-
 [Phe9]angiotensin I 37°C, pH 7.5, mutant enzyme R1098Q Homo sapiens
0.044
-
 [Arg10]angiotensin I 37°C, pH 7.5, wild-type enzyme Homo sapiens
0.046
-
 [Arg10]angiotensin I 37°C, pH 7.5, mutant enzyme Y1096F Homo sapiens
0.052
-
 angiotensin I 37°C, pH 7.5, wild-type enzyme Homo sapiens
0.056
-
 [Phe9]angiotensin I 37°C, pH 7.5, mutant enzyme Y1096F Homo sapiens
0.1
-
 angiotensin I 37°C, pH 7.5, mutant enzyme R1098Q Homo sapiens
0.22
-
 [Arg10]angiotensin I 37°C, pH 7.5, mutant enzyme R1098Q Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
angiotensin I + H2O
-
 Homo sapiens angiotensin II + His-Leu
-
 ?
bradykinin + H2O
-
 Homo sapiens ?
-
 ?
[Arg10]angiotensin I + H2O
-
 Homo sapiens ?
-
 ?
[Phe9,Arg10]angiotensin I + H2O
-
 Homo sapiens ?
-
 ?
[Phe9]angiotensin I + H2O
-
 Homo sapiens ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.5
-
 [Phe9,Arg10]angiotensin I 37°C, pH 7.5, mutant enzyme K1087A Homo sapiens
52
-
 angiotensin I 37°C, pH 7.5, wild-type enzyme Homo sapiens
57
-
 [Arg10]angiotensin I 37°C, pH 7.5, mutant enzyme Y1096F Homo sapiens
60
-
 angiotensin I 37°C, pH 7.5, mutant enzyme R1098Q Homo sapiens
100
-
 [Arg10]angiotensin I 37°C, pH 7.5, mutant enzyme R1098Q Homo sapiens
110
-
 [Phe9]angiotensin I 37°C, pH 7.5, mutant enzyme Y1096F Homo sapiens
220
-
 [Phe9,Arg10]angiotensin I 37°C, pH 7.5, mutant enzyme Y1096F Homo sapiens
460
-
 [Arg10]angiotensin I 37°C, pH 7.5, wild-type enzyme Homo sapiens
550
-
 [Phe9]angiotensin I 37°C, pH 7.5, wild-type enzyme Homo sapiens
1400
-
 [Phe9,Arg10]angiotensin I 37°C, pH 7.5, wild-type enzyme Homo sapiens
1500
-
 [Phe9]angiotensin I 37°C, pH 7.5, mutant enzyme R1098Q Homo sapiens
1700
-
 [Phe9,Arg10]angiotensin I 37°C, pH 7.5, mutant enzyme R1098Q Homo sapiens