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Literature summary for 3.4.15.1 extracted from

  • Moiseeva, N.A.; Binevski, P.V.; Baskin, II; Palyulin, V.A.; Kost, O.A.
    Role of two chloride-binding sites in functioning of testicular angiotensin-converting enzyme (2005), Biochemistry (Moscow), 70, 1167-1172.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Cl- kcat increases with increasing KCl concentrations, reaches a maximum at about 300 mM KCl, and the begins to decrease. At relatively low concentrations chloride anions activate the C-domain of the enzyme, but at high concentrations chloride inhibits the enzyme activity. Presence of at least two chloride-binding sites in the C-domain of bovine enzyme: binding of chloride to one of the sites causes activation of the enzyme, whereas chloride binding to the second site results in inhibition of the enzymatic activity Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
Cl- kcat increases with increasing KCl concentrations, reaches a maximum at about 300 mM KCl, and the begins to decrease. At relatively low concentrations chloride anions activate the C-domain of the enzyme, but at high concentrations chloride inhibits the enzyme activity. Presence of at least two chloride-binding sites in the C-domain of bovine enzyme: binding of chloride to one of the sites causes activation of the enzyme, whereas chloride binding to the second site results in inhibition of the enzymatic activity Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
benzyloxycarbonyl-Phe-His-Leu pH 7.5 Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
testis
-
Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-Phe-His-Leu + H2O
-
Bos taurus benzyloxycarbonyl-Phe + His-Leu
-
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Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
21.5
-
benzyloxycarbonyl-Phe-His-Leu pH 7.5 Bos taurus