Literature summary for 3.4.14.9 extracted from

Pal, A.; Kraetzner, R.; Gruene, T.; Grapp, M.; Schreiber, K.; Gronborg, M.; Urlaub, H.; Becker, S.; Asif, A.R.; Gaertner, J.; Sheldrick, G.M.; Steinfeld, R.
Structure of tripeptidyl-peptidase I provides insight into the molecular basis of late infantile neuronal ceroid lipofuscinosis (2009), J. Biol. Chem., 284, 3976-3984.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in HEK-293 cells	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
fully-glycosylated TPP1 precursor, hanging drop vapour diffusion method, using 7% PEG4000, 0.02 M zinc sulfate, 0.1 M sodium acetate, pH 5.0, and 0.1 M ammonium sulfate	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
G284V	the mutation probably compromises the active center and results in loss of proteolytic activity	Homo sapiens
G473R	the mutation probably compromises the active center and results in loss of proteolytic activity	Homo sapiens
K428N	no apparent conformational destabilization is observed for the missense mutation	Homo sapiens
N286S	the substitution results in loss of one glycosylation site, which leads to almost complete loss of protease activity	Homo sapiens
Q248P	the mutation probably compromises the active center and results in loss of proteolytic activity	Homo sapiens
R266Q	no apparent conformational destabilization is observed for the missense mutation	Homo sapiens
S475L	the mutation probably compromises the active center and results in loss of proteolytic activity	Homo sapiens
V216M	no apparent conformational destabilization is observed for the missense mutation	Homo sapiens
V277M	the mutation probably compromises the active center and results in loss of proteolytic activity	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	contains one Ca2+ ion	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
46000	-	autoprocessed TPP1, SDS-PAGE	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	O14773	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	TPP1 requires at least partial glycosylation for in vitro autoprocessing and proteolytic activity	Homo sapiens

Purification (Commentary)

Purification (Comment)	Organism
HisTrap HP column chromatography	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-Ala-L-Ala-L-Phe-7-amido-4-methylcoumarin + H2O	-	Homo sapiens	L-Ala-L-Ala-L-Phe + 7-amino-4-methylcoumarin	-	?

Synonyms

Synonyms	Comment	Organism
Tpp1	-	Homo sapiens

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Release 2023.1 (January 2023)