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Literature summary for 3.4.14.9 extracted from

  • Golabek, A.A.; Dolzhanskaya, N.; Walus, M.; Wisniewski, K.E.; Kida, E.
    Prosegment of tripeptidyl peptidase I is a potent, slow-binding inhibitor of its cognate enzyme (2008), J. Biol. Chem., 283, 16497-16504.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.14.9

Cloned(Commentary)

Cloned (Comment) Organism
TPP I prosegment is expressed in Escherichia coli BL21 (DE3) cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
G77R the mutation is associated with classic late infantile neuronal ceroid lipofuscinosis Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
prosegment of tripeptidyl peptidase I wild type prosegment of tripeptidyl peptidase I (176 amino acid residues) is a potent, slow-binding inhibitor of its parent enzyme with an overall inhibition constant in the low nanomolar range, in the presence of the prosegment (at 0.001 mM) leads from a significant to complete inhibition of TPP I activity at pH between 3.5 and 5.5 with IC 50 ranging from more than 0.016 mM to 0.000043 mM at pH from 3.5 to 5.5 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22000
-
 TPP I prosegment, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+ affinity resin chromatography and heparin agarose affinity chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Ala-L-Ala-L-Phe-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens L-Ala-L-Ala-L-Phe + 7-amino-4-methylcoumarin
-
 ?

Synonyms

Synonyms Comment Organism
TPP I
-
 Homo sapiens
tripeptidyl peptidase I
-
 Homo sapiens

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.5
-
 TPP I is an acidic protease that is quickly inactivated under alkaline pH conditions, in the absence of prosegment, the enzyme is quickly denatured with a rate constant of around 0.107/min, amounting to a half-life of 6.472 min, whereas in the presence of the prosegment, the inactivation rate is reduced to about 0.035/min, and approximately 84% of the activity is preserved after over 2 h of incubation Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0001581
-
 prosegment of tripeptidyl peptidase I in sodium acetate buffer (50 mM, pH 5.0), 0.05% Triton X-100 Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0001292
-
 in sodium acetate buffer (50 mM, pH 5.0), 0.05% Triton X-100 Homo sapiens prosegment of tripeptidyl peptidase I