Cloned (Comment) | Organism |
---|---|
expression in silkworm pupae | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D165A | inactive mutant | Homo sapiens |
D81A | not expressed in Sf9 cells | Homo sapiens |
E77A | very low activity with Ala-Arg-Phe-p-nitrophenylalanyl-Arg-Leu | Homo sapiens |
S280A | inactive mutant | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetylpepstatin | 1 mM, 14% inhibition | Homo sapiens | |
Ala-Ala-Phe-chloromethylketone | 0.1 mM, complete inhibition | Homo sapiens | |
chymostatin | 1 mM, 47% inhibition | Homo sapiens | |
N-acetyl-Ile-Ala-phenylalaninal | 1 mM, 88% inhibition | Homo sapiens | |
N-acetyl-Ile-Pro-phenylalaninal | 1 mM, 38% inhibition | Homo sapiens | |
Tyrostatin | 1 mM, 19% inhibition | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00402 | - |
Ala-Arg-Phe-p-nitrophenylalanyl-Arg-Leu | pH 4.0, 37°C, wild-type enzyme | Homo sapiens | |
0.00509 | - |
Ala-Asp-Phe-p-nitrophenylalanyl-Arg-Leu | pH 4.0, 37°C, wild-type enzyme | Homo sapiens | |
0.0148 | - |
Ala-Ala-Phe-p-nitrophenylalanyl-Arg-Leu | pH 4.0, 37°C, wild-type enzyme | Homo sapiens | |
0.0193 | - |
Arg-Ala-Phe-p-nitrophenylalanyl-Arg-Leu | pH 4.0, 37°C, wild-type enzyme | Homo sapiens | |
0.0422 | - |
Ala-His-Phe-p-nitrophenylalanyl-Arg-Leu | pH 4.0, 37°C, wild-type enzyme | Homo sapiens | |
0.0483 | - |
Ala-Ser-Phe-p-nitrophenylalanyl-Arg-Leu | pH 4.0, 37°C, wild-type enzyme | Homo sapiens | |
0.108 | - |
Ser-Ala-Phe-p-nitrophenylalanyl-Arg-Leu | pH 4.0, 37°C, wild-type enzyme | Homo sapiens | |
0.11 | - |
Asp-Ala-Phe-p-nitrophenylalanyl-Arg-Leu | pH 4.0, 37°C, wild-type enzyme | Homo sapiens | |
0.123 | - |
Ala-Arg-Phe-p-nitrophenylalanyl-Arg-Leu | pH 4.0, 37°C, mutant enzyme E77A | Homo sapiens | |
0.167 | - |
His-Ala-Phe-p-nitrophenylalanyl-Arg-Leu | pH 4.0, 37°C, wild-type enzyme | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
x * 46000, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | TPP-I is synthesized as a 66000 Da inactive precursor consisting of a 195 residue prepro-peptide and 368 residue mature enzyme. Most of the precursor proteins are converted to a 46000 Da form by post-translational proteolysis | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | Ala-Ala-Phe + 7-amino-4-methylcoumarin | - |
? | |
Ala-Ala-Phe-p-nitrophenylalanyl-Arg-Leu + H2O | - |
Homo sapiens | Ala-Ala-Phe + p-nitrophenylalanyl-Arg-Leu | - |
? | |
Ala-Arg-Phe-p-nitrophenylalanyl-Arg-Leu + H2O | - |
Homo sapiens | Ala-Arg-Phe + p-nitrophenylalanyl-Arg-Leu | - |
? | |
Ala-Asp-Phe-p-nitrophenylalanyl-Arg-Leu + H2O | - |
Homo sapiens | Ala-Asp-Phe + p-nitrophenylalanyl-Arg-Leu | - |
? | |
Ala-His-Phe-p-nitrophenylalanyl-Arg-Leu + H2O | - |
Homo sapiens | Ala-His-Phe + p-nitrophenylalanyl-Arg-Leu | - |
? | |
Ala-Ser-Phe-p-nitrophenylalanyl-Arg-Leu + H2O | - |
Homo sapiens | Ala-Ser-Phe + p-nitrophenylalanyl-Arg-Leu | - |
? | |
Arg-Ala-Phe-p-nitrophenylalanyl-Arg-Leu + H2O | - |
Homo sapiens | Arg-Ala-Phe + p-nitrophenylalanyl-Arg-Leu | - |
? | |
Asp-Ala-Phe-p-nitrophenylalanyl-Arg-Leu + H2O | - |
Homo sapiens | Asp-Ala-Phe + p-nitrophenylalanyl-Arg-Leu | - |
? | |
His-Ala-Phe-p-nitrophenylalanyl-Arg-Leu + H2O | - |
Homo sapiens | His-Ala-Phe + p-nitrophenylalanyl-Arg-Leu | - |
? | |
Ser-Ala-Phe-p-nitrophenylalanyl-Arg-Leu + H2O | - |
Homo sapiens | Ser-Ala-Phe + p-nitrophenylalanyl-Arg-Leu | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 46000, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
CLN2 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0124 | - |
Ala-Arg-Phe-p-nitrophenylalanyl-Arg-Leu | pH 4.0, 378°C, mutant enzyme E77A | Homo sapiens | |
0.91 | - |
Arg-Ala-Phe-p-nitrophenylalanyl-Arg-Leu | pH 4.0, 37°C, wild-type enzyme | Homo sapiens | |
4.7 | - |
Ser-Ala-Phe-p-nitrophenylalanyl-Arg-Leu | pH 4.0, 37°C, wild-type enzyme | Homo sapiens | |
11 | - |
Ala-Asp-Phe-p-nitrophenylalanyl-Arg-Leu | pH 4.0, 37°C, wild-type enzyme | Homo sapiens | |
11.8 | - |
Ala-Arg-Phe-p-nitrophenylalanyl-Arg-Leu | pH 4.0, 37°C, wild-type enzyme | Homo sapiens | |
13.7 | - |
His-Ala-Phe-p-nitrophenylalanyl-Arg-Leu | pH 4.0, 37°C, wild-type enzyme | Homo sapiens | |
18.5 | - |
Asp-Ala-Phe-p-nitrophenylalanyl-Arg-Leu | pH 4.0, 37°C, wild-type enzyme | Homo sapiens | |
26.4 | - |
Ala-His-Phe-p-nitrophenylalanyl-Arg-Leu | pH 4.0, 37°C, wild-type enzyme | Homo sapiens | |
30.7 | - |
Ala-Ser-Phe-p-nitrophenylalanyl-Arg-Leu | pH 4.0, 37°C, wild-type enzyme | Homo sapiens | |
34.5 | - |
Ala-Ala-Phe-p-nitrophenylalanyl-Arg-Leu | pH 4.0, 37°C, wild-type enzyme | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
- |
Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 4.8 | pH 3.0: about 30% of maximal activity, pH 4.8: about 50% of maximal activity | Homo sapiens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2.5 | 5 | stable | Homo sapiens |