Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3,4-dichloroisocoumarin | weak | Homo sapiens | |
diethyldicarbonate | - |
Homo sapiens | |
diisopropylfluorophosphate | at high concentration | Homo sapiens | |
additional information | no inhibition by pepstatin; no inhibition: EDTA; no inhibition: Phe-Pro-Arg chloromethylketone, inhibitors of cysteine proteinases or metalloproteinases, iodoacetamide, trans-epoxysuccinyl-L-leucinamido(4-guanidino)butane (i.e. E-64), mercaptoethanol, bestatin, soybean trypsin inhibitor, phenylchloromethyl ketone | Homo sapiens | |
p-tosyl-L-Lys chloromethyl ketone | weak | Homo sapiens | |
Phenyl-N-phenylphosphoamidochloridate | weak | Homo sapiens | |
phenylmethylsulfonyl fluoride | weak | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
lysosome | - |
Homo sapiens | 5764 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
x * 48000, SDS-PAGE | Homo sapiens |
700000 | - |
gel filtration in the absence or presence of 4 M urea | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | involved in degradation of bone collagen | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
osteoclastoma cell | bone tumor | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | Ala-Ala-Phe + 7-amino-4-methylcoumarin | - |
? | |
Gly-Pro-Met 2-naphthylamide + H2O | - |
Homo sapiens | Gly-Pro-Met + 2-naphthylamine | - |
? | |
additional information | succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin, succinyl-Gly-Pro-Leu 4-methylcoumarin 7-amide, Gly-Pro-7-amido-4-methylcoumarin, Gly-7-amido-4-methylcoumarin, Pro-7-amido-4-methylcoumarin, Met-7-amido-4-methylcoumarin, Ala 7-amido-4-methylcoumarin, Phe-7-amido-4-methylcoumarin, or Leu-7-amido-4-methylcoumarin | Homo sapiens | ? | - |
? | |
additional information | absolute requirement for unsubstituted amino-terminus | Homo sapiens | ? | - |
? | |
additional information | involved in degradation of bone collagen | Homo sapiens | ? | - |
? | |
synthetic collagen-like polypetides + H2O | - |
Homo sapiens | Gly-Pro-Xaa tripeptides | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 48000, SDS-PAGE | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 5.5 | hydrolysis of Gly-Pro-Met 2-naphthylamide or Ala-Ala-Phe 4-methylcoumarin 7-amide | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 8 | about half-maximal activity at pH 3 and 8, hydrolysis of Gly-Pro-Met 2-naphthylamide | Homo sapiens |
3.5 | 6.5 | about half-maximal activity at pH 3.5 and 6.5, hydrolysis of Ala-Ala-Phe 4-methylcoumarin 7-amide | Homo sapiens |