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Literature summary for 3.4.14.6 extracted from

  • Eng, F.W.H.T.
    Dipeptidyl tetrapeptide hyrolase, a new enzyme with dipeptidyl ligase activity (1984), Can. J. Biochem. Cell Biol., 62, 516-528.
No PubMed abstract available
Search for more literature for 3.4.14.6

Activating Compound

Activating Compound Comment Organism Structure
thiols activate, strict requirement for reduced sulfhydryl group Brassica oleracea

Inhibitors

Inhibitors Comment Organism Structure
diisopropylfluorophosphate alone slightly and in combination with p-hydroxymercuribenzoate strong Brassica oleracea
dithiothreitol slight Brassica oleracea
p-hydroxymercuribenzoate
-
 Brassica oleracea
puromycin 0.005 M: no effect, 0.025 M: complete inhibition, Ala-Gly-Ala-Gly synthesis Brassica oleracea

Organism

Organism UniProt Comment Textmining
Brassica oleracea
-
 cabbage
-

Purification (Commentary)

Purification (Comment) Organism
partial Brassica oleracea

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00867
-
-
 Brassica oleracea
0.0267
-
-
 Brassica oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Gly
-
 Brassica oleracea Ala-Gly-Ala-Gly + H2O
-
 r
Ala-Gly-Ala-Gly + H2O
-
 Brassica oleracea 2 Ala-Gly
-
 r
Gly-Gly + H2O
-
 Brassica oleracea Gly-Gly-Gly-Gly
-
 r
Gly-Gly-Gly-Gly + H2O
-
 Brassica oleracea Gly-Gly
-
 r
Gly-L-Ala + H2O
-
 Brassica oleracea Gly-Ala-Gly-Ala
-
 ?
L-Ala-L-Ala + H2O
-
 Brassica oleracea Ala-Ala-Ala-Ala
-
 ?
L-Met-Gly + H2O very slowly Brassica oleracea ?
-
 ?
additional information tetrapeptides are formed from Ala-Ala, Gly-Gly, Ala-Gly and Gly-Ala without prior activation of the carboxyl group, condensation reaction: substrate specificity for a dipeptide with either two Ala residues or one Ala and one Gly residue, not hydrolyzed: pentaglycine, no condensation reaction with: Ser-Gly, Ala-Ala-Gly, Gly-Gly-Ala, Gly-Gly-Gly, Gly-Gly-leu, beta-Ala-Gly, Gly-leu, D-Ala-L-Ala, Gly-D-Ala Brassica oleracea ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Brassica oleracea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
-
 Brassica oleracea

pH Range

pH Minimum pH Maximum Comment Organism
6 8.8 pH 6.0: about 50% of activity maximum, pH 8.8: about 5% of activity maximum Brassica oleracea