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Literature summary for 3.4.14.5 extracted from

  • Jobin, M.C.; Martinez, G.; Motard, J.; Gottschalk, M.; Grenier, D.
    Cloning, purification, and enzymatic properties of dipeptidyl peptidase IV from the swine pathogen Streptococcus suis (2005), J. Bacteriol., 187, 795-799.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.14.5

Activating Compound

Activating Compound Comment Organism Structure
aminoethyl-benzene sulfonyl fluoride hydrochloride 1 mM, 18% activation Streptococcus suis
N-(trans-epoxysuccinyl)-L-leucine-4-guanidinobutylamide 0.01 mM, 25% activation Streptococcus suis

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Streptococcus suis

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 10 mM, 34% inhibition Streptococcus suis
3,4-dichloroisocoumarin 0.1 mM, 18% inhibition Streptococcus suis
Diprotin A 1 mM, 71% inhibition Streptococcus suis
Hg2+ 1 mM, 70% inhibition Streptococcus suis
iodoacetamide 0.01 mM, 19% inhibition Streptococcus suis
SDS 35 mM, complete inhibition Streptococcus suis
Triton X-100 16 mM, 22% inhibition Streptococcus suis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.26
-
 Gly-Pro-4-nitroanilide
-
 Streptococcus suis

Organism

Organism UniProt Comment Textmining
Streptococcus suis Q673U2
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptococcus suis

Storage Stability

Storage Stability Organism
-20°C, stable to prolonged storage Streptococcus suis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Ala-4-nitroanilide + H2O 36% of the activity with Gly-Pro-4-nitroanilide Streptococcus suis Ala-Ala + 4-nitroaniline
-
 ?
Ala-Pro-4-nitroanilide + H2O 80% of the activity with Gly-Pro-4-nitroanilide Streptococcus suis Ala-Pro + 4-nitroaniline
-
 ?
Arg-Pro-4-nitroanilide + H2O 42% of the activity with Gly-Pro-4-nitroanilide Streptococcus suis Arg-Pro + 4-nitroaniline
-
 ?
Asp-Pro-4-nitroanilide + H2O 11% of the activity with Gly-Pro-4-nitroanilide Streptococcus suis Asp-Pro + 4-nitroaniline
-
 ?
Gly-Pro-4-nitroanilide + H2O
-
 Streptococcus suis Gly-Pro + 4-nitroaniline
-
 ?
Lys-Pro-4-nitroanilide + H2O 51% of the activity with Gly-Pro-4-nitroanilide Streptococcus suis Lys-Pro + 4-nitroaniline
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Streptococcus suis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
24 45 24°C: 52% of maximal activity, 45°C: 78% of maximal activity Streptococcus suis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
 24 h, 86% loss of activity, diluted preparation of recombinant enzyme Streptococcus suis
10
-
 24 h, 92% loss of activity, diluted preparation of recombinant enzyme Streptococcus suis
24
-
 24 h, 93% loss of activity, diluted preparation of recombinant enzyme Streptococcus suis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Streptococcus suis

pH Range

pH Minimum pH Maximum Comment Organism
6 9 pH 6: 61% of maximal activity, pH 9: 54% of maximal activity Streptococcus suis