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Literature summary for 3.4.14.5 extracted from

  • Kumagai, Y.; Konishi, K.; Gomi, T.; Yagishita, H.; Yajima, A.; Yoshikawa, M.
    Enzymatic properties of dipeptidyl aminopeptidase IV produced by the periodontal pathogen Porphyromonas gingivalis and its participation in virulence (2000), Infect. Immun., 68, 716-724.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.14.5

Activating Compound

Activating Compound Comment Organism Structure
additional information not affected by dithiothreitol Porphyromonas gingivalis

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli Porphyromonas gingivalis

Protein Variants

Protein Variants Comment Organism
D702A site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme Porphyromonas gingivalis
H743A site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme Porphyromonas gingivalis
additional information compared to the wild-type strain, an enzyme-deficient mutant strain leads to less severe infections and a faster recovery from the infection when injected into mice Porphyromonas gingivalis
S624A site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme Porphyromonas gingivalis

Inhibitors

Inhibitors Comment Organism Structure
additional information not affected by dithiothreitol Porphyromonas gingivalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.21
-
 Gly-Pro-4-nitroanilide recombinant enzyme, pH 7.5, 25°C Porphyromonas gingivalis
0.28
-
 Gly-Pro-4-nitroanilide native enzyme, pH 7.5, 25°C Porphyromonas gingivalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
74000
-
 2 * 74000, native enzyme, SDS-PAGE Porphyromonas gingivalis
140000
-
 native enzyme, gel filtration Porphyromonas gingivalis

Organism

Organism UniProt Comment Textmining
Porphyromonas gingivalis O66223 periodontal pathogen, strain W38, gene dpp
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 1000fold to homogeneity, and recombinant enzyme from Escherichia coli Porphyromonas gingivalis

Reaction

Reaction Comment Organism Reaction ID
release of an N-terminal dipeptide, Xaa-Yaa-/-Zaa-, from a polypeptide, preferentially when Yaa is Pro, provided Zaa is neither Pro nor hydroxyproline conserved residues Ser624, Asp702, and His734 form a catalytic triad and are involved in the catalytic reaction, Ser593 is likely to be the catalytic serine of the serine protease Porphyromonas gingivalis
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.002
-
 below, mutant enzymes Porphyromonas gingivalis
15
-
 purified native enzyme Porphyromonas gingivalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Gly-Pro-4-nitroanilide + H2O
-
 Porphyromonas gingivalis Gly-Pro + 4-nitroaniline
-
 ?
additional information the enzyme does not bind to adenosine deaminase, EC 3.5.4.4 Porphyromonas gingivalis ?
-
 ?
QPDAINAP + H2O peptide sequence corresponds to the N-terminus of human MCP1 protein Porphyromonas gingivalis Gln-Pro + DAINAP
-
 ?
SPYSSDTT + H2O peptide sequence corresponds to the N-terminus of human RANTES protein Porphyromonas gingivalis Ser-Pro + YSSDTT
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 74000, native enzyme, SDS-PAGE Porphyromonas gingivalis

Synonyms

Synonyms Comment Organism
dipeptidyl aminopeptidase IV
-
 Porphyromonas gingivalis
DPPIV
-
 Porphyromonas gingivalis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Porphyromonas gingivalis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 native enzyme, 30 min, 70% remaining activity Porphyromonas gingivalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 native enzyme Porphyromonas gingivalis