Cloned (Comment) | Organism |
---|---|
overexpression of the soluble His-tagged protein in Pichia pastoris strain X-33 under control of AOX1 promotor, removal of the membrane anchor, residues 1-36, secretion of the recombinant enzyme extracellular part into the cell culture medium and the periplasm | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
isoleucyl-(2)-cyanopyrrolidine | - |
Homo sapiens | |
isoleucyl-thiazolidine | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.063 | - |
Gly-Pro-7-amido-4-methylcoumarin | pH 7.6, 30°C, recombinant soluble enzyme | Homo sapiens | |
0.165 | - |
Gly-Pro-4-nitroanilide | pH 7.6, 30°C, recombinant soluble enzyme | Homo sapiens | |
2.74 | - |
Ala-Ala-4-nitroanilide | pH 7.6, 30°C, recombinant soluble enzyme | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
plasma membrane | type II transmembrane protein | Homo sapiens | 5886 | - |
soluble | - |
Homo sapiens | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
103600 | - |
2 * 103600, recombinant soluble enzyme, MALDI-TOF mass spectrometry | Homo sapiens |
210000 | - |
about, recombinant soluble enzyme, gel filtration | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | enzyme is involved in diabetes mellitus, AIDS, and immunoregulatory processes | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | heterologous glycosylation, several isozymes | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant soluble His-tagged extracellular part of the enzyme from Pichia pastoris culture medium, 534fold | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
release of an N-terminal dipeptide, Xaa-Yaa-/-Zaa-, from a polypeptide, preferentially when Yaa is Pro, provided Zaa is neither Pro nor hydroxyproline | serine protease | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
32.3 | - |
purified recombinant soluble enzyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ala-Ala-4-nitroanilide + H2O | - |
Homo sapiens | Ala-Ala + 4-nitroaniline | - |
? | |
Gly-Pro-4-nitroanilide + H2O | - |
Homo sapiens | Gly-Pro + 4-nitroaniline | - |
? | |
Gly-Pro-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | Gly-Pro + 7-amino-4-methylcoumarin | - |
? | |
additional information | enzyme is involved in diabetes mellitus, AIDS, and immunoregulatory processes | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 103600, recombinant soluble enzyme, MALDI-TOF mass spectrometry | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
dipeptidyl peptidase IV | - |
Homo sapiens |
DP IV | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000038 | - |
isoleucyl-(2)-cyanopyrrolidine | pH 7.6, 30°C, recombinant soluble enzyme | Homo sapiens | |
0.000085 | - |
isoleucyl-thiazolidine | pH 7.6, 30°C, recombinant soluble enzyme | Homo sapiens |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Homo sapiens | several isozymes due to heterologous glycosylation, isoelectric focusing | - |
5.47 |