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Literature summary for 3.4.14.5 extracted from

  • Miyakawa, H.; Hashimoto, I.; Nakamura, T.; Ishibashi, N.; Shimamura, S.; Igoshi, K.
    Purification and characterization of an X-prolyl dipeptidyl aminopeptidase from Lactobacillus helveticus LHE-511 (1994), Milchwissenschaft, 49, 670-673.
No PubMed abstract available
Search for more literature for 3.4.14.5

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
 Lactobacillus helveticus
Cu2+
-
 Lactobacillus helveticus
diisopropyl fluorophosphate
-
 Lactobacillus helveticus
dithiothreitol
-
 Lactobacillus helveticus
EDTA
-
 Lactobacillus helveticus
Hg2+
-
 Lactobacillus helveticus
NEM
-
 Lactobacillus helveticus
PCMB
-
 Lactobacillus helveticus
phenylmethylsulfonyl fluoride
-
 Lactobacillus helveticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
87000
-
 1 * 87000, SDS-PAGE Lactobacillus helveticus
93000
-
 gel filtration Lactobacillus helveticus

Organism

Organism UniProt Comment Textmining
Lactobacillus helveticus
-
 LHE-511
-
Lactobacillus helveticus LHE-511
-
 LHE-511
-

Purification (Commentary)

Purification (Comment) Organism
-
 Lactobacillus helveticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
20.2
-
-
 Lactobacillus helveticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Ala-4-nitroanilide + H2O
-
 Lactobacillus helveticus Ala-Ala + 4-nitroaniline
-
 ?
Ala-Ala-4-nitroanilide + H2O
-
 Lactobacillus helveticus LHE-511 Ala-Ala + 4-nitroaniline
-
 ?
Ala-Pro-4-nitroanilide + H2O
-
 Lactobacillus helveticus Ala-Pro + 4-nitroaniline
-
 ?
Ala-Pro-4-nitroanilide + H2O
-
 Lactobacillus helveticus LHE-511 Ala-Pro + 4-nitroaniline
-
 ?
Arg-Pro-4-nitroanilide + H2O
-
 Lactobacillus helveticus Arg-Pro + 4-nitroaniline
-
 ?
Arg-Pro-4-nitroanilide + H2O
-
 Lactobacillus helveticus LHE-511 Arg-Pro + 4-nitroaniline
-
 ?
Arg-Pro-Pro + H2O
-
 Lactobacillus helveticus ?
-
 ?
Gly-Pro-4-nitroanilide + H2O
-
 Lactobacillus helveticus Gly-Pro + 4-nitroaniline
-
 ?
Gly-Pro-4-nitroanilide + H2O
-
 Lactobacillus helveticus LHE-511 Gly-Pro + 4-nitroaniline
-
 ?
Gly-Pro-Arg + H2O
-
 Lactobacillus helveticus Gly-Pro + Arg
-
 ?
Gly-Pro-Arg + H2O
-
 Lactobacillus helveticus LHE-511 Gly-Pro + Arg
-
 ?
Ile-Pro-Ile + H2O
-
 Lactobacillus helveticus ?
-
 ?
Val-Pro-Leu + H2O
-
 Lactobacillus helveticus ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 87000, SDS-PAGE Lactobacillus helveticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Lactobacillus helveticus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 30 min, about 40% loss of activity Lactobacillus helveticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Lactobacillus helveticus

pH Range

pH Minimum pH Maximum Comment Organism
5 8 pH 5.0: about 50% of maximal activity, pH 8.0: about 60% of maximal activity Lactobacillus helveticus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 7.5 37°C, 60 min, stable Lactobacillus helveticus