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Literature summary for 3.4.14.5 extracted from

  • Ohkubo, I.; Huang, K.; Ochiai, Y.; Takagaki, M.; Kani, K.
    Dipeptidyl peptidase IV from porcine seminal plasma: purification, characterization, and N-terminal amino acid sequence (1994), J. Biochem., 116, 1182-1186.
    View publication on PubMed
Search for more literature for 3.4.14.5

General Stability

General Stability Organism
resistant against several cycles of repeated freezing and thawing Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
4-(2-aminoethyl)-benzenesulfonyl fluoride
-
 Sus scrofa
Cd2+
-
 Sus scrofa
Cu2+ 1 mM, 17% inhibition Sus scrofa
diisopropyl fluorophosphate
-
 Sus scrofa
Diprotin A
-
 Sus scrofa
Hg2+
-
 Sus scrofa
Ni2+
-
 Sus scrofa
phenylmethylsulfonyl fluoride
-
 Sus scrofa
Zn2+
-
 Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
105000
-
 3 * 105000, SDS-PAGE in presence of 2-mercaptoethanol Sus scrofa
115000
-
 3 * 115000, SDS-PAGE in absence of 2-mercaptoethanol Sus scrofa
290000
-
 non-denaturing PAGE Sus scrofa
310000
-
 gel filtration Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
seminal plasma
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8
-
-
 Sus scrofa

Storage Stability

Storage Stability Organism
-30°C, 20 mM Tris-HCl, pH 8.0, stable for several months Sus scrofa
4°C, 20 mM Tris-HCl, pH 8.0, stable for at least 6 weeks Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Gly-Pro-4-methylcoumarin 7-amide + H2O
-
 Sus scrofa Gly-Pro + 7-amino-4-methylcoumarin
-
 ?
Lys-Ala-4-methylcoumarin 7-amide + H2O
-
 Sus scrofa Lys-Ala + 7-amino-4-methylcoumarin
-
 ?

Subunits

Subunits Comment Organism
trimer 3 * 115000, SDS-PAGE in absence of 2-mercaptoethanol Sus scrofa
trimer 3 * 105000, SDS-PAGE in presence of 2-mercaptoethanol Sus scrofa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 30 min, stable up to Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Sus scrofa

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 9.5 4°C, 24 h, stable Sus scrofa