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Literature summary for 3.4.14.5 extracted from

  • Yoshimoto, T.; Kita, T.; Ichinose, M.; Tsuru, D.
    Dipeptidyl aminopeptidase IV from porcine pancreas (1982), J. Biochem., 92, 275-282.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline slight Sus scrofa
diisopropyl fluorophosphate
-
Sus scrofa
EDTA slight Sus scrofa
iodoacetate slight Sus scrofa
NEM slight Sus scrofa
PCMB slight Sus scrofa
phenylmethylsulfonyl fluoride slight Sus scrofa
Zn2+
-
Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.57
-
Gly-Pro-7-amido-4-methylcoumarin
-
Sus scrofa
0.63
-
Gly-Pro-4-nitroanilide
-
Sus scrofa
0.82
-
Ala-Ala-2-naphthylamide
-
Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
115000
-
2 * 115000, SDS-PAGE Sus scrofa
230000
-
gel filtration Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
pancreas
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
9.29
-
-
Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Ala-2-naphthylamide + H2O
-
Sus scrofa Ala-Ala + 2-naphthylamine
-
?
Gly-Pro-2-naphthylamide + H2O
-
Sus scrofa Gly-Pro + 2-naphthylamine
-
?
Gly-Pro-4-methylcoumarin 7-amide + H2O
-
Sus scrofa Gly-Pro + 7-amino-4-methylcoumarin
-
?
Gly-Pro-4-nitroanilide + H2O
-
Sus scrofa Gly-Pro + 4-nitroaniline
-
?

Subunits

Subunits Comment Organism
dimer 2 * 115000, SDS-PAGE Sus scrofa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
pH 7.0, 1 h Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
hydrolysis of Gly-Pro-2-naphthylamide Sus scrofa

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 9 37°C, 1 h, stable Sus scrofa