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Literature summary for 3.4.14.5 extracted from
- Soluble dipeptidyl peptidase IV from terminal differentiated rat epidermal cells: purification and its activity on synthetic and natural peptides (1988), Arch. Biochem. Biophys., 266, 369-376.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | 1 mM | Rattus norvegicus |  |
| Co2+ | 1 mM | Rattus norvegicus | |
| diisopropyl fluorophosphate | - |
Rattus norvegicus | |
| Diprotin A | - |
Rattus norvegicus | |
| Hg2+ | 1 mM | Rattus norvegicus | |
| Ni2+ | 1 mM | Rattus norvegicus | |
| phenylmethylsulfonyl fluoride | slight | Rattus norvegicus | |
| Zn2+ | 1 mM | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.025 | - |
Tyr-Pro-Phe-Val-Glu-Pro-Ile | - |
Rattus norvegicus | |
| 0.05 | - |
Arg-Pro-Lys-Pro | - |
Rattus norvegicus | |
| 0.05 | - |
Tyr-Pro-Phe-Val-Glu-Pro-Ile | - |
Rattus norvegicus | |
| 0.05 | - |
Tyr-Pro-Phe-Pro-Gly | - |
Rattus norvegicus | |
| 0.05 | - |
Tyr-Pro-Phe | - |
Rattus norvegicus | |
| 0.05 | - |
Tyr-Pro-Phe-Pro | - |
Rattus norvegicus | |
| 0.09 | - |
Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 | - |
Rattus norvegicus | |
| 0.15 | - |
Arg-Pro-Lys-Pro-Gln-Gln-Phe | - |
Rattus norvegicus | |
| 0.18 | - |
Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly | - |
Rattus norvegicus | |
| 0.3 | - |
Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 | - |
Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 90000 | - |
2 * 90000, SDS-PAGE | Rattus norvegicus |
| 190000 | - |
gel filtration | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| side-chain modification | glycoprotein | Rattus norvegicus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| epidermis | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 31.9 | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Arg-Pro-Lys-Pro + H2O | - |
Rattus norvegicus | ? | - |
? | |
| Arg-Pro-Lys-Pro-Gln-Gln-Phe + H2O | - |
Rattus norvegicus | ? | - |
? | |
| Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly | - |
Rattus norvegicus | Arg-Pro + Lys-Pro-Gln-Gln-Phe-Phe-Gly | - |
? | |
| Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly + H2O | - |
Rattus norvegicus | ? | - |
? | |
| Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 + H2O | - |
Rattus norvegicus | Arg-Pro + Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 | - |
? | |
| Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH3 + H2O | - |
Rattus norvegicus | ? | - |
? | |
| beta-casomorphin + H2O | beta-casomorphin from human, i.e. Tyr-Pro-Phe-Val-Glu-Pro-Ileu | Rattus norvegicus | ? | - |
? | |
| beta-casomorphin + H2O | beta-casomorphin from bovine, i.e. Tyr-Pro-Phe-Val-Glu-Pro-Ile | Rattus norvegicus | ? | - |
? | |
| Gly-Pro-4-nitroanilide + H2O | - |
Rattus norvegicus | Gly-Pro + 4-nitroaniline | - |
? | |
| Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 + H2O | - |
Rattus norvegicus | ? | - |
? | |
| substance P + H2O | - |
Rattus norvegicus | Arg-Pro + Lys-Pro + ? | - |
? | |
| Tyr-Pro-Phe + H2O | - |
Rattus norvegicus | ? | - |
? | |
| Tyr-Pro-Phe-Pro + H2O | - |
Rattus norvegicus | ? | - |
? | |
| Tyr-Pro-Phe-Pro-Gly + H2O | - |
Rattus norvegicus | ? | - |
? | |
| Tyr-Pro-Phe-Val-Glu-Pro-Ile + H2O | - |
Rattus norvegicus | Tyr-Pro + Phe-Val-Glu-Pro-Ile | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 90000, SDS-PAGE | Rattus norvegicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
pH 6.0-9.5, stable for 2 h | Rattus norvegicus |
| 45 | - |
10 min, stable | Rattus norvegicus |
| 60 | - |
10 min, 46% loss of activity | Rattus norvegicus |
| 70 | - |
10 min, complete loss of activity | Rattus norvegicus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 50.3 | - |
Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 | - |
Rattus norvegicus | |
| 60.3 | - |
Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly | - |
Rattus norvegicus | |
| 62.3 | - |
Arg-Pro-Lys-Pro-Gln-Gln-Phe-Gly | - |
Rattus norvegicus | |
| 66.1 | - |
Arg-Pro-Lys-Pro | - |
Rattus norvegicus | |
| 71.4 | - |
Tyr-Pro-Phe-Val-Glu-Pro-Ile | - |
Rattus norvegicus | |
| 87.5 | - |
Tyr-Pro-Phe | - |
Rattus norvegicus | |
| 88.3 | - |
Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 | - |
Rattus norvegicus | |
| 90.8 | - |
Tyr-Pro-Phe-Pro | - |
Rattus norvegicus | |
| 102 | - |
Tyr-Pro-Phe-Pro-Gly | - |
Rattus norvegicus | |
| 103 | - |
Tyr-Pro-Phe-Val-Glu-Pro-Ileu | - |
Rattus norvegicus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
hydrolysis of Gly-Pro-4-nitroanilide, substance P or beta-casomorphin | Rattus norvegicus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9.5 | at 37°C stable for 2 h, at 4°C stable for 3 days | Rattus norvegicus |
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