Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.14.5 extracted from

  • Tsakalidou, E.; Anastasiou, R.; Papadimitriou, K.; Manopoulou, E.; Kalantzopoulos, G.
    Purification and characterisation of an intracellular X-prolyl-dipeptidyl aminopeptidase from Streptococus thermophilus ACA-DC 4 (1997), J. Biotechnol., 59, 203-211.
    View publication on PubMed
Search for more literature for 3.4.14.5

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Streptococcus thermophilus
iodoacetate
-
 Streptococcus thermophilus
phenylmethylsulfonyl fluoride
-
 Streptococcus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1
-
 Gly-Pro-p-nitroanilide
-
 Streptococcus thermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
 2 * 80000, SDS-PAGE Streptococcus thermophilus
160000
-
 gel filtration Streptococcus thermophilus

Organism

Organism UniProt Comment Textmining
Streptococcus thermophilus
-
 ACA-DC 4
-
Streptococcus thermophilus ACA-DC 4
-
 ACA-DC 4
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptococcus thermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
285
-
-
 Streptococcus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-4-nitroanilide + H2O 1% of the activity with Gly-Pro-4-nitroanilide Streptococcus thermophilus Ala + 4-nitroaniline
-
 ?
Ala-Pro-Gly + H2O 72% of the activity with Gly-Pro-4-nitroanilide Streptococcus thermophilus Ala-Pro + Gly
-
 ?
Arg-4-nitroanilide + H2O 2% of the activity with Gly-Pro-4-nitroanilide Streptococcus thermophilus Arg + 4-nitroaniline
-
 ?
Arg-4-nitroanilide + H2O 2% of the activity with Gly-Pro-4-nitroanilide Streptococcus thermophilus ACA-DC 4 Arg + 4-nitroaniline
-
 ?
Arg-Pro-4-nitroanilide + H2O 55% of the activity with Gly-Pro-4-nitroanilide Streptococcus thermophilus Arg-Pro + 4-nitroaniline
-
 ?
Arg-Pro-4-nitroanilide + H2O 55% of the activity with Gly-Pro-4-nitroanilide Streptococcus thermophilus ACA-DC 4 Arg-Pro + 4-nitroaniline
-
 ?
Gly-Phe-4-nitroanilide + H2O 1% of the activity with Gly-Pro-4-nitroanilide Streptococcus thermophilus Gly-Phe + 4-nitroaniline
-
 ?
Gly-Pro-4-nitroanilide + H2O
-
 Streptococcus thermophilus Gly-Pro + 4-nitroaniline
-
 ?
Gly-Pro-4-nitroanilide + H2O
-
 Streptococcus thermophilus ACA-DC 4 Gly-Pro + 4-nitroaniline
-
 ?
Gly-Pro-Arg-Pro + H2O as active as with Gly-Pro-4-nitroanilide Streptococcus thermophilus ?
-
 ?
Gly-Pro-Arg-Pro + H2O as active as with Gly-Pro-4-nitroanilide Streptococcus thermophilus ACA-DC 4 ?
-
 ?
Ile-Pro-Ile + H2O 34% of the activity with Gly-Pro-4-nitroanilide Streptococcus thermophilus ?
-
 ?
Phe-4-nitroanilide + H2O 7% of the activity with Gly-Pro-4-nitroanilide Streptococcus thermophilus Phe + 4-nitroaniline
-
 ?
Phe-4-nitroanilide + H2O 7% of the activity with Gly-Pro-4-nitroanilide Streptococcus thermophilus ACA-DC 4 Phe + 4-nitroaniline
-
 ?
Pro-4-nitroanilide + H2O 2% of the activity with Gly-Pro-4-nitroanilide Streptococcus thermophilus Pro + 4-nitroaniline
-
 ?
Val-Pro-Leu + H2O 83% of the activity with Gly-Pro-4-nitroanilide Streptococcus thermophilus ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 80000, SDS-PAGE Streptococcus thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Streptococcus thermophilus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 60 10°C: 17% of maximal activity, about about 40% of maximal activity at 25°C and at 60°C Streptococcus thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 10 min, stable Streptococcus thermophilus
60
-
 1 min, in absence of substrate, 96% loss of activity Streptococcus thermophilus
70
-
 1 min, complete loss of activity Streptococcus thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Streptococcus thermophilus

pH Range

pH Minimum pH Maximum Comment Organism
5 9 pH 5.0: about 15% of maximal activity, pH 9.0: about 85% of maximal activity Streptococcus thermophilus