Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 1.0 mM, 14 mM 40°C, partial | Lactobacillus helveticus | |
Cu2+ | 0.1-2.5 mM | Lactobacillus helveticus | |
diisopropyl fluorophosphate | 1.0 mM, 2 h, 40°C, complete inhibition | Lactobacillus helveticus | |
EDTA | 1.0 mM, 40°C, 20 h, partial | Lactobacillus helveticus | |
Hg2+ | 0.1-2.5 mM | Lactobacillus helveticus | |
iodoacetate | 1.0 mM, partial | Lactobacillus helveticus | |
NEM | 1.0 mM, partial | Lactobacillus helveticus | |
PCMB | 1.0 mM, 15 min, 40°C, complete inhibition | Lactobacillus helveticus | |
phenylmethylsulfonyl fluoride | 1.0 mM, partial | Lactobacillus helveticus | |
Zn2+ | 0.1-2.5 mM | Lactobacillus helveticus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 0.1-2.5 mM, activates | Lactobacillus helveticus | |
K+ | 10-50 mM, activates | Lactobacillus helveticus | |
Mg2+ | 2.5 mM, activates | Lactobacillus helveticus | |
Na+ | 10-50 mM, activates | Lactobacillus helveticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72000 | - |
non-denaturing PAGE | Lactobacillus helveticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactobacillus helveticus | - |
CNRZ 32 | - |
Lactobacillus helveticus CNRZ 32 | - |
CNRZ 32 | - |
Purification (Comment) | Organism |
---|---|
- |
Lactobacillus helveticus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Lactobacillus helveticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ala-Ala-4-nitroanilide + H2O | 2% of the activity with Gly-Pro-4-nitroanilide | Lactobacillus helveticus | Ala-Ala + 4-nitroaniline | - |
? | |
Ala-Ala-4-nitroanilide + H2O | 2% of the activity with Gly-Pro-4-nitroanilide | Lactobacillus helveticus CNRZ 32 | Ala-Ala + 4-nitroaniline | - |
? | |
Ala-Pro-Gly + H2O | 11% of the activity with Gly-Pro-4-nitroanilide | Lactobacillus helveticus | Ala-Pro + Gly | - |
? | |
Ala-Pro-Gly + H2O | 11% of the activity with Gly-Pro-4-nitroanilide | Lactobacillus helveticus CNRZ 32 | Ala-Pro + Gly | - |
? | |
Arg-Pro-4-nitroanilide + H2O | 130% of the activity with Gly-Pro-4-nitroanilide | Lactobacillus helveticus | Arg-Pro + 4-nitroaniline | - |
? | |
Arg-Pro-4-nitroanilide + H2O | 130% of the activity with Gly-Pro-4-nitroanilide | Lactobacillus helveticus CNRZ 32 | Arg-Pro + 4-nitroaniline | - |
? | |
Gly-Pro-4-nitroanilide + H2O | - |
Lactobacillus helveticus | Gly-Pro + 4-nitroaniline | - |
? | |
Gly-Pro-4-nitroanilide + H2O | - |
Lactobacillus helveticus CNRZ 32 | Gly-Pro + 4-nitroaniline | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 72000, SDS-PAGE | Lactobacillus helveticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Lactobacillus helveticus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | 60 | about 40% of maximal activity at 5°C and at 60°C | Lactobacillus helveticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
hydrolysis of Gly-Pro-p-nitroanilide | Lactobacillus helveticus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8.5 | pH 5.0: about 50% of maximal activity, pH 8.5: about 60% of maximal activity | Lactobacillus helveticus |