Protein Variants | Comment | Organism |
---|---|---|
Leu453del | activity of mutant Cu(II)-del-DPP III, in which Leu453 is deleted from the metal-binding motif, is only 1-2% of the enzyme activity of del-DPP. The EPR spectra of Cu(II) del-DPP III do not change in the presence of excess Lys-Ala-beta-naphthylamide. The deletion of Leu453 from the HELLGH motif of rat DPP III leads to a complete loss of flexibility in the ligand geometry around the cupric ions | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
L-Lys-L-Ala-2-naphthylamide | wild-type in presence of Cu2+, pH 7.4, 25°C | Rattus norvegicus | |
0.12 | - |
L-Lys-L-Ala-2-naphthylamide | wild-type, pH 7.4, 25°C | Rattus norvegicus | |
0.2 | - |
L-Lys-L-Ala-2-naphthylamide | mutant Leu453del, pH 7.4, 25°C | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | activity of the cupric derivative for Lys-Ala-beta-naphthylamide is about 30% of that of the wild-type zinc enzyme. The enzyme activity of mutant Cu(II)-del-DPP III, in which Leu453 is deleted from the metal-binding motif, is only 1-2% of the enzyme activity of del-DPP III. The EPR spectra of Cu(II) del-DPP III do not change in the presence of excess Lys-Ala-beta-naphthylamide. The deletion of Leu453 from the HELLGH motif of rat DPP III leads to a complete loss of flexibility in the ligand geometry around the cupric ions | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Lys-L-Ala-2-naphthylamide + H2O | activity of the cupric derivative for Lys-Ala-beta-naphthylamide is about 30% of that of the wild-type enzyme | Rattus norvegicus | L-Lys-L-Ala + 2-naphthylamine | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
L-Lys-L-Ala-2-naphthylamide | wild-type in presence of Cu2+, pH 7.4, 25°C | Rattus norvegicus | |
1.1 | - |
L-Lys-L-Ala-2-naphthylamide | mutant Leu453del, pH 7.4, 25°C | Rattus norvegicus | |
1.4 | - |
L-Lys-L-Ala-2-naphthylamide | wild-type, pH 7.4, 25°C | Rattus norvegicus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.5 | - |
L-Lys-L-Ala-2-naphthylamide | mutant Leu453del, pH 7.4, 25°C | Rattus norvegicus | |
5.7 | - |
L-Lys-L-Ala-2-naphthylamide | wild-type in presence of Cu2+, pH 7.4, 25°C | Rattus norvegicus | |
12 | - |
L-Lys-L-Ala-2-naphthylamide | wild-type, pH 7.4, 25°C | Rattus norvegicus |