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Literature summary for 3.4.14.4 extracted from

  • Dhanda, S.; Singh, H.; Singh, J.; Singh, T.P.
    Functional characterization and specific effects of various peptides on enzymatic activity of a DPP-III homologue from goat brain (2008), J. Enzyme Inhib. Med. Chem., 23, 174-181.
    View publication on PubMed
Search for more literature for 3.4.14.4

Activating Compound

Activating Compound Comment Organism Structure
dimethyl sulfoxide the activity progressively increases with increase in dimethyl sulfoxide concentration up to 5% (v/v) Capra hircus

Inhibitors

Inhibitors Comment Organism Structure
3,3'-dithiobis(6-nitrobenzoic acid) 13% residual activity at 2.5 mM, beta-mercaptoethanol and dithiothreitlol completely restores enzyme activity Capra hircus
cysteine
-
 Capra hircus
dimethyl sulfoxide inhibitory effect with more than 5% (v/v) dimethyl sulfoxide Capra hircus
dithioerythreitol
-
 Capra hircus
dithiothreitol
-
 Capra hircus
EDTA 49.2% residual activity at 1 mM Capra hircus
ethanol the enzyme loses about half of its activity at 5% (v/v) ethanol Capra hircus
glutathione
-
 Capra hircus
Gly-L-Glu 36% inhibition at 1 mM Capra hircus
Gly-L-Phe 46% inhibition at 1 mM Capra hircus
Gly-L-Phe-L-Leu non-competitive inhibitor, 82% inhibition at 1 mM Capra hircus
Hg2+ 11.1% residual activity at 0.1 mM Capra hircus
K+ 82.0% residual activity at 0.1 mM Capra hircus
L-Ala-L-Ala-L-Ala 4% inhibition at 1 mM Capra hircus
L-Ala-L-Ala-L-Ala-L-Ala 58% inhibition at 0.5 mM Capra hircus
L-Arg-L-Phe 56% inhibition at 1 mM Capra hircus
L-Arg-L-Phe-L-Ala competitive inhibitor, 99% inhibition at 1 mM Capra hircus
L-Arg-L-Trp 46% inhibition at 1 mM Capra hircus
L-Arg-L-Val 49% inhibition at 1 mM Capra hircus
L-Asp-L-Ala 25% inhibition at 1 mM Capra hircus
L-His-L-Leu 76% inhibition at 1 mM Capra hircus
L-His-L-Lys 41% inhibition at 1 mM Capra hircus
L-His-L-Phe 46% inhibition at 1 mM Capra hircus
L-His-L-Pro 16% inhibition at 1 mM Capra hircus
L-Ile-L-His 34% inhibition at 1 mM Capra hircus
L-Leu-L-Arg complete inhibition at 0.5 mM Capra hircus
L-Leu-L-Trp competitive inhibitor, 90% inhibition at 1 mM Capra hircus
L-Leu-L-Trp-L-Met competitive inhibitor, 92% inhibition at 1 mM Capra hircus
L-Leu-L-Trp-L-Met-L-Arg-L-Phe-L-Ala competitive inhibitor, complete inhibition at 1 mM Capra hircus
L-Lys-L-Ala 23% inhibition at 1 mM Capra hircus
L-Met-L-Arg 99% inhibition at 1 mM Capra hircus
L-Phe-L-Ala 41% inhibition at 1 mM Capra hircus
L-Phe-L-Arg 75% inhibition at 1 mM Capra hircus
L-Phe-L-Leu 58% inhibition at 1 mM Capra hircus
L-Phe-L-Met 85% inhibition at 1 mM Capra hircus
L-Pro-L-Arg-OH 54% inhibition at 1 mM Capra hircus
L-Pro-L-Glu 34% inhibition at 1 mM Capra hircus
L-Pro-L-Phe 56% inhibition at 1 mM Capra hircus
L-Ser-L-Met 32% inhibition at 1 mM Capra hircus
L-Trp-L-Met competitive inhibitor, 94% inhibition at 1 mM Capra hircus
L-Trp-L-Met-L-Asp-L-Phe 82% inhibition at 1 mM Capra hircus
L-tryptophyl-L-methionyl-L-alpha-aspartyl-L-phenylalaninamide competitive inhibition Capra hircus
L-Tyr-Gly 31% inhibition at 1 mM Capra hircus
L-Tyr-Gly-Gly-L-Phe 53% inhibition at 1 mM Capra hircus
L-Tyr-L-Ile 72% inhibition at 1 mM Capra hircus
L-Val-L-Tyr competitive inhibitor, 84% inhibition at 1 mM Capra hircus
Leu-enkephalin i.e. L-Tyr-Gly-Gly-L-Phe-L-Leu, 92% inhibition at 1 mM, competitive inhibitor Capra hircus
Li+ 73.0% residual activity at 0.1 mM Capra hircus
Met-enkephalin i.e. L-Tyr-Gly-Gly-L-Phe-L-Met, 61% inhibition at 0.5 mM, competitive inhibitor Capra hircus
additional information not inhibited by beta-mercaptoethanol Capra hircus
Ni2+ 12.4% residual activity at 0.1 mM Capra hircus
thioglycolic acid
-
 Capra hircus
Urea sensitive to very low concentrations of urea Capra hircus
Zn2+ 50.3% residual activity at 1 mM Capra hircus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 174.4% relative activity at 0.1 mM Capra hircus

Organism

Organism UniProt Comment Textmining
Capra hircus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Capra hircus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Capra hircus
-

Storage Stability

Storage Stability Organism
-20°C, in 50 mM Tris-HCl, pH 7.5, 1 and 6 months, 38.15% and 99.75% loss of activity Capra hircus
-20°C, in 50 mM Tris-HCl, pH 7.5, in the presence of 10% (v/v) glycerol, 1 and 6 months, 26.5% and 92% loss of activity Capra hircus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Arg-L-Arg-4-methoxy-2-naphthylamide + H2O
-
 Capra hircus L-Arg-L-Arg + 4-methoxy-2-naphthylamine
-
 ?

Synonyms

Synonyms Comment Organism
dipeptidyl peptidase III
-
 Capra hircus
DPP-III
-
 Capra hircus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.004
-
 L-Arg-L-Phe-L-Ala in 50 mM Tris-HCl, pH 8.5, with 100 mM NaCl and 1 mM beta-mercaptoethanol, at 37°C Capra hircus
0.007
-
 L-Leu-L-Trp-L-Met-L-Arg-L-Phe-L-Ala in 50 mM Tris-HCl, pH 8.5, with 100 mM NaCl and 1 mM beta-mercaptoethanol, at 37°C Capra hircus
0.015
-
 Gly-L-Phe-L-Leu in 50 mM Tris-HCl, pH 8.5, with 100 mM NaCl and 1 mM beta-mercaptoethanol, at 37°C Capra hircus
0.031
-
 L-Leu-L-Trp-L-Met in 50 mM Tris-HCl, pH 8.5, with 100 mM NaCl and 1 mM beta-mercaptoethanol, at 37°C Capra hircus
0.039
-
 L-Leu-L-Trp in 50 mM Tris-HCl, pH 8.5, with 100 mM NaCl and 1 mM beta-mercaptoethanol, at 37°C Capra hircus
0.055
-
 L-tryptophyl-L-methionyl-L-alpha-aspartyl-L-phenylalaninamide in 50 mM Tris-HCl, pH 8.5, with 100 mM NaCl and 1 mM beta-mercaptoethanol, at 37°C Capra hircus
0.09
-
 Leu-enkephalin in 50 mM Tris-HCl, pH 8.5, with 100 mM NaCl and 1 mM beta-mercaptoethanol, at 37°C Capra hircus
0.13
-
 Met-enkephalin in 50 mM Tris-HCl, pH 8.5, with 100 mM NaCl and 1 mM beta-mercaptoethanol, at 37°C Capra hircus
0.25
-
 L-Val-L-Tyr in 50 mM Tris-HCl, pH 8.5, with 100 mM NaCl and 1 mM beta-mercaptoethanol, at 37°C Capra hircus
0.38
-
 L-Trp-L-Met in 50 mM Tris-HCl, pH 8.5, with 100 mM NaCl and 1 mM beta-mercaptoethanol, at 37°C Capra hircus