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Literature summary for 3.4.14.4 extracted from

  • Dhanda, S.; Singh, H.; Singh, J.; Singh, T.P.
    Isolation, purification and characterization of a DPP-III homologue from goat brain (2007), Protein Expr. Purif., 52, 297-305.
    View publication on PubMed
Search for more literature for 3.4.14.4

Inhibitors

Inhibitors Comment Organism Structure
1-ethyl-3(3-dimethyl-amino-propyl)-carbodiimide*HCl 1.0 mM inhibitor, 29% inhibition, 5.0 mM inhibitor, 64% inhibition Capra hircus
2,2'-dithiodipyridine 1.0 mM inhibitor, 32% inhibition, 2.5 mM inhibitor, 98% inhibition Capra hircus
2,4,6-trinitrobenzene sufonic acid trihydrate 1.0 mM inhibitor, 52% inhibition, 2.5 mM inhibitor, 89% inhibition Capra hircus
alpha1 anti-trypsin 1.0 mM inhibitor, 61% inhibition Capra hircus
Aprotinin 1.0 mM inhibitor, 59% inhibition, 5.0 mM inhibitor, 99% inhibition Capra hircus
benzamidine HCl 1.0 mM inhibitor, 29% inhibition, 5.0 mM inhibitor, 61% inhibition Capra hircus
DTNB 1.0 mM inhibitor, 54% inhibition, 2.5 mM inhibitor, 87% inhibition Capra hircus
EDTA 1.0 mM inhibitor, 50% inhibition, 5.0 mM inhibitor, 64% inhibition Capra hircus
Egg white trypsin inhibitor 1.0 mM inhibitor, 34% inhibition Capra hircus
iodoacetamide 1.0 mM inhibitor, 27% inhibition, 5.0 mM inhibitor, 42% inhibition Capra hircus
iodoacetic acid 1.0 mM inhibitor, 25% inhibition, 5.0 mM inhibitor, 48% inhibition Capra hircus
leupeptin 1.0 mM inhibitor, 32% inhibition Capra hircus
N-ethylmaleimide 1.0 mM inhibitor, 29% inhibition, 2.5 mM inhibitor, 88% inhibition, 5.0 mM inhibitor, 96% inhibition Capra hircus
N-hydroxy-succinimide 1.0 mM inhibitor, 30% inhibition, 5.0 mM inhibitor, 51% inhibition Capra hircus
o-phenanthroline 1.0 mM inhibitor, 46% inhibition, 2.5 mM inhibitor, 85% inhibition, 5.0 mM inhibitor, 96% inhibition Capra hircus
p-aminophenyl mercuric acetate 1.0 mM inhibitor, 42% inhibition, 2.5 mM inhibitor, 94% inhibition Capra hircus
p-hydroxymercuriphenyl-sulfonic acid 1.0 mM inhibitor, 22% inhibition, 2.5 mM inhibitor, 96% inhibition Capra hircus
p-nitrocatecholsulfate 1.0 mM inhibitor, 37% inhibition, 5.0 mM inhibitor, 58% inhibition Capra hircus
Pb(CH3COO)2 1.0 mM inhibitor, 53% inhibition, 2.5 mM inhibitor, 97% inhibition Capra hircus
pepstatin A 5.0 mM inhibitor, 92% inhibition Capra hircus
PMSF 1.0 mM inhibitor, 53% inhibition, 2.5 mM inhibitor, 87% inhibition Capra hircus
puromycin 1.0 mM inhibitor, 30% inhibition, 5.0 mM inhibitor, 58% inhibition Capra hircus
Soybean trypsin inhibitor 1.0 mM inhibitor, 64% inhibition, 2.5 mM inhibitor, 76% inhibition Capra hircus
TLCK 1.0 mM inhibitor, 27% inhibition, 5.0 mM inhibitor, 63% inhibition Capra hircus
TPCK 1.0 mM inhibitor, 75% inhibition, 5.0 mM inhibitor, 88% inhibition Capra hircus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.039
-
 Arg-Arg-4-methoxy-beta-naphthylamide
-
 Capra hircus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
69000
-
 determined by SDS-PAGE, gel filtration and MALDI-TOF analysis Capra hircus

Organism

Organism UniProt Comment Textmining
Capra hircus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DPP from goat brain is purified by several steps, including acid precipitation, ammonium sulfate fractionation, chromatography on a Sephadex G-100 column, cation, anion and adsorption chromatography Capra hircus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Capra hircus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0001
-
 purification step, homogenate Capra hircus
0.0004
-
 purification step, acid precipitation Capra hircus
0.0012
-
 purification step, ammonium sulfate fractionation, 35-65% pellet Capra hircus
0.0092
-
 purification step, gel filtration chromatography on a Sephadex G-100 column Capra hircus
0.0126
-
 purification step, cation exchange chromatography on a CM Sephadex A-50 column Capra hircus
0.0652
-
 purification step, anion exchange chromatography on a DEAE Sephadex A-50 column Capra hircus
0.107
-
 purification step, adsorption chromatography on a hydroxyapatite column Capra hircus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Ala-beta-naphthylamide + H2O 10.0% activity Capra hircus Ala-Ala + beta-naphthylamine
-
 ?
Arg-Arg-4-methoxy-beta-naphthylamide + H2O activity assay, 100% activity Capra hircus Arg-Arg + 4-methoxy-beta-naphthylamine
-
 ?
Asp-Ala-beta-naphthylamide + H2O 0% activity Capra hircus Asp-Ala + beta-naphthylamine
-
 ?
Asp-Arg-beta-naphthylamide + H2O 1.5% activity Capra hircus Asp-Arg + beta-naphthylamine
-
 ?
Gly-Ala-beta-naphthylamide + H2O 3.2% activity Capra hircus Gly-Ala + beta-naphthylamine
-
 ?
Gly-Arg-beta-naphthylamide + H2O 11.8% activity Capra hircus Gly-Arg + beta-naphthylamine
-
 ?
Gly-Phe-beta-naphthylamide + H2O 2.4% activity Capra hircus Gly-Phe + beta-naphthylamine
-
 ?
Gly-Pro-4-methoxy-beta-naphthylamide + H2O 0% activity Capra hircus Gly-Pro + 4-methoxy-beta-naphthylamine
-
 ?
His-Phe-beta-naphthylamide + H2O 0% activity Capra hircus His-Phe + beta-naphthylamine
-
 ?
His-Ser-beta-naphthylamide + H2O 0% activity Capra hircus His-Ser + beta-naphthylamine
-
 ?
Leu-Ala-beta-naphthylamide + H2O 5.0% activity Capra hircus Leu-Ala + beta-naphthylamine
-
 ?
Lys-Ala-4-methoxy-beta-naphthylamide + H2O 5.0% activity Capra hircus Lys-Ala + 4-methoxy-beta-naphthylamine
-
 ?
Phe-Arg-beta-naphthylamide + H2O 17.6% activity Capra hircus Phe-Arg + beta-naphthylamine
-
 ?
Ser-Met-beta-naphthylamide + H2O 0% activity Capra hircus Ser-Met + beta-naphthylamine
-
 ?
Ser-Tyr-beta-naphthylamide + H2O 0% activity Capra hircus Ser-Tyr + beta-naphthylamine
-
 ?

Subunits

Subunits Comment Organism
monomer
-
 Capra hircus

Synonyms

Synonyms Comment Organism
dipeptidyl peptidase III
-
 Capra hircus
DPP III
-
 Capra hircus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 activity assay Capra hircus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40
-
 the enzyme shows a considerable heat stability up to 40°C, 30% activity is lost at 45°C Capra hircus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.039
-
 Arg-Arg-4-methoxy-beta-naphthylamide
-
 Capra hircus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 activity assay Capra hircus

pH Range

pH Minimum pH Maximum Comment Organism
6 11 the enzyme has 50% activity at pH 6 in the lower range and at pH 11 in the higher range Capra hircus

pI Value

Organism Comment pI Value Maximum pI Value
Capra hircus
-
-
 4.5