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Literature summary for 3.4.14.2 extracted from

  • Fukasawa, K.; Fukasawa, K.M.; Hiraoka, B.Y.; Harada, M.
    Purification and properties of dipeptidyl peptidase II from rat kidney (1983), Biochim. Biophys. Acta, 745, 6-11.
    View publication on PubMed
Search for more literature for 3.4.14.2

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Rattus norvegicus
diisopropyl fluorophosphate
-
 Rattus norvegicus
Hg2+
-
 Rattus norvegicus
Pb2+
-
 Rattus norvegicus
phenylmethylsulfonyl fluoride
-
 Rattus norvegicus
puromycin
-
 Rattus norvegicus
Tris
-
 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.487
-
 Ala-Ala-4-nitroanilide
-
 Rattus norvegicus
0.555
-
 Lys-Ala-2-naphthylamide
-
 Rattus norvegicus
1.09
-
 Gly-Pro-Ala
-
 Rattus norvegicus
1.67
-
 Ala-Ala-Ala
-
 Rattus norvegicus
5.55
-
 Gly-Ala-Ala
-
 Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64000
-
 2 * 64000, SDS-PAGE Rattus norvegicus
130000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Rattus norvegicus hydrolyzes specifically N-terminal X-alanine or X-proline from their respective arylamides and from tripeptides or tetrapeptides, but not from pentapeptides ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
66.2
-
-
 Rattus norvegicus

Storage Stability

Storage Stability Organism
-20°C, stable for at least 3 months Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(Ala)3 + H2O
-
 Rattus norvegicus ?
-
 ?
(Ala)4 + H2O
-
 Rattus norvegicus ?
-
 ?
Ala-Ala-4-nitroanilide + H2O
-
 Rattus norvegicus Ala-Ala + 4-nitroaniline
-
 ?
Glu-Pro-4-nitroanilide + H2O
-
 Rattus norvegicus Glu-Pro + 4-nitroaniline
-
 ?
Gly-Ala-Ala + H2O
-
 Rattus norvegicus Gly-Ala + Ala
-
 ?
Gly-Pro-4-nitroanilide + H2O
-
 Rattus norvegicus Gly-Pro + 4-nitroaniline
-
 ?
Gly-Pro-Ala + H2O
-
 Rattus norvegicus Gly-Pro + Ala
-
 ?
Gly-Pro-Leu-Gly + H2O
-
 Rattus norvegicus ?
-
 ?
Lys-Ala-2-naphthylamide + H2O
-
 Rattus norvegicus Lys-Ala + 2-naphthylamine
-
 ?
Lys-Pro-4-nitroanilide + H2O
-
 Rattus norvegicus Lys-Pro + 4-nitroaniline
-
 ?
additional information hydrolyzes specifically N-terminal X-alanine or X-proline from their respective arylamides and from tripeptides or tetrapeptides, but not from pentapeptides Rattus norvegicus ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 64000, SDS-PAGE Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
103
-
 Gly-Ala-Ala
-
 Rattus norvegicus
109
-
 Gly-Pro-4-nitroanilide
-
 Rattus norvegicus
120
-
 Gly-Pro-Ala
-
 Rattus norvegicus
121
-
 Ala-Ala-Ala
-
 Rattus norvegicus
314
-
 Ala-Ala-4-nitroanilide
-
 Rattus norvegicus
536
-
 Lys-Ala-2-naphthylamide
-
 Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
 hydrolysis of tripeptides Rattus norvegicus
5.5
-
 hydrolysis of arylamides Rattus norvegicus