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Literature summary for 3.4.14.13 extracted from

  • Schmidt, D.M.; Hubbard, B.K.; Gerlt, J.A.
    Evolution of enzymatic activities in the enolase superfamily: functional assignment of unknown proteins in Bacillus subtilis and Escherichia coli as L-Ala-D/L-Glu epimerases (2001), Biochemistry, 40, 15707-15715.
    View publication on PubMed
Search for more literature for 3.4.14.13

Cloned(Commentary)

Cloned (Comment) Organism
-
 Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
 L-Ala-gamma-D-Glu-L-Lys-D-Ala pH and temperature not specified in the publication Bacillus subtilis
0.29
-
 L-Ala-gamma-D-Glu-L-Lys pH and temperature not specified in the publication Bacillus subtilis
0.31
-
 L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala pH and temperature not specified in the publication Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala + H2O Bacillus subtilis the enzyme from is involved in the recycling of the murein peptide L-Ala-D-Glu + L-Lys-D-Ala-D-Ala
-
 ?
L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala + H2O Bacillus subtilis 168 the enzyme from is involved in the recycling of the murein peptide L-Ala-D-Glu + L-Lys-D-Ala-D-Ala
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis O35010
-
-
Bacillus subtilis 168 O35010
-
-

Purification (Commentary)

Purification (Comment) Organism
His-tagged enzyme Bacillus subtilis

Storage Stability

Storage Stability Organism
-20°C, 40% glycerol, significant loss of activity Bacillus subtilis
4°C, stable in absence of glycerol for 48h Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Ala-gamma-D-Glu-L-Lys + H2O
-
 Bacillus subtilis L-Ala-D-Glu + L-Lys
-
 ?
L-Ala-gamma-D-Glu-L-Lys + H2O
-
 Bacillus subtilis 168 L-Ala-D-Glu + L-Lys
-
 ?
L-Ala-gamma-D-Glu-L-Lys-D-Ala + H2O
-
 Bacillus subtilis L-Ala-D-Glu + L-Lys-D-Ala
-
 ?
L-Ala-gamma-D-Glu-L-Lys-D-Ala + H2O
-
 Bacillus subtilis 168 L-Ala-D-Glu + L-Lys-D-Ala
-
 ?
L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala + H2O the enzyme from is involved in the recycling of the murein peptide Bacillus subtilis L-Ala-D-Glu + L-Lys-D-Ala-D-Ala
-
 ?
L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala + H2O the enzyme is specific for the gamma-D-Glu-L-Lys bond. No activity with L-Ala-D-Glu-L-Lys-D-Ala-D-Ala or L-Ala-gamma-L-Glu-L-Lys-D-Ala-D-Ala Bacillus subtilis L-Ala-D-Glu + L-Lys-D-Ala-D-Ala
-
 ?
L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala + H2O the enzyme from is involved in the recycling of the murein peptide Bacillus subtilis 168 L-Ala-D-Glu + L-Lys-D-Ala-D-Ala
-
 ?
L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala + H2O the enzyme is specific for the gamma-D-Glu-L-Lys bond. No activity with L-Ala-D-Glu-L-Lys-D-Ala-D-Ala or L-Ala-gamma-L-Glu-L-Lys-D-Ala-D-Ala Bacillus subtilis 168 L-Ala-D-Glu + L-Lys-D-Ala-D-Ala
-
 ?

Synonyms

Synonyms Comment Organism
gamma-D-glutamyl-L-lysine endopeptidase
-
 Bacillus subtilis
YkfC
-
 Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Bacillus subtilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2
-
 L-Ala-gamma-D-Glu-L-Lys pH and temperature not specified in the publication Bacillus subtilis
2.6
-
 L-Ala-gamma-D-Glu-L-Lys-D-Ala pH and temperature not specified in the publication Bacillus subtilis
5.7
-
 L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala pH and temperature not specified in the publication Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 assay at Bacillus subtilis

General Information

General Information Comment Organism
physiological function the enzyme is involved in the recycling of the murein peptide Bacillus subtilis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.1
-
 L-Ala-gamma-D-Glu-L-Lys pH and temperature not specified in the publication Bacillus subtilis
18
-
 L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala pH and temperature not specified in the publication Bacillus subtilis
22
-
 L-Ala-gamma-D-Glu-L-Lys-D-Ala pH and temperature not specified in the publication Bacillus subtilis